1lka: Difference between revisions

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{{Seed}}
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{{STRUCTURE_1lka|  PDB=1lka  |  SCENE=  }}  
{{STRUCTURE_1lka|  PDB=1lka  |  SCENE=  }}  


'''Porcine Pancreatic Elastase/Ca-Complex'''
===Porcine Pancreatic Elastase/Ca-Complex===




==Overview==
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Porcine pancreatic elastase has been crystallized at slightly acidic pH under two similar but slightly different conditions. Diffraction data were collected at a wavelength of 1.5 A to a maximum resolution of 1.7 A. Both difference electron-density maps and anomalous difference electron-density maps suggest that in crystals grown from a sodium sulfate solution PPE binds Na(+) in its metal-binding site. In contrast, PPE binds Ca(2+) in crystals grown from a solution containing sodium citrate and calcium chloride. This observation is in contradiction to most PPE structures reported in the PDB. In addition to the metal-binding site, up to three other binding sites, which appear to be anion-binding sites, could be identified based on the observed anomalous intensity differences.
The line below this paragraph, {{ABSTRACT_PUBMED_12198296}}, adds the Publication Abstract to the page
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{{ABSTRACT_PUBMED_12198296}}


==About this Structure==
==About this Structure==
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[[Category: Chloride binding]]
[[Category: Chloride binding]]
[[Category: Chymotrypsin-fold]]
[[Category: Chymotrypsin-fold]]
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