1lkd: Difference between revisions

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[[Image:1lkd.jpg|left|200px]]
{{Seed}}
[[Image:1lkd.png|left|200px]]


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{{STRUCTURE_1lkd|  PDB=1lkd  |  SCENE=  }}  
{{STRUCTURE_1lkd|  PDB=1lkd  |  SCENE=  }}  


'''CRYSTAL STRUCTURE OF 2,3-DIHYDROXYBIPHENYL 1,2-DIOXYGENASE (DHBD) COMPLEXED WITH 2',6'-DICL DIHYDROXYBIPHENYL (DHB)'''
===CRYSTAL STRUCTURE OF 2,3-DIHYDROXYBIPHENYL 1,2-DIOXYGENASE (DHBD) COMPLEXED WITH 2',6'-DICL DIHYDROXYBIPHENYL (DHB)===




==Overview==
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The microbial degradation of polychlorinated biphenyls (PCBs) provides the potential to destroy these widespread, toxic and persistent environmental pollutants. For example, the four-step upper bph pathway transforms some of the more than 100 different PCBs found in commercial mixtures and is being engineered for more effective PCB degradation. In the critical third step of this pathway, 2,3-dihydroxybiphenyl (DHB) 1,2-dioxygenase (DHBD; EC 1.13.11.39) catalyzes aromatic ring cleavage. Here we demonstrate that ortho-chlorinated PCB metabolites strongly inhibit DHBD, promote its suicide inactivation and interfere with the degradation of other compounds. For example, k(cat)(app) for 2',6'-diCl DHB was reduced by a factor of approximately 7,000 relative to DHB, and it bound with sufficient affinity to competitively inhibit DHB cleavage at nanomolar concentrations. Crystal structures of two complexes of DHBD with ortho-chlorinated metabolites at 1.7 A resolution reveal an explanation for these phenomena, which have important implications for bioremediation strategies.
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{{ABSTRACT_PUBMED_12415290}}


==About this Structure==
==About this Structure==
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[[Category: Non-heme iron]]
[[Category: Non-heme iron]]
[[Category: Pcb biodegradation]]
[[Category: Pcb biodegradation]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 00:00:18 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul 2 21:17:48 2008''

Revision as of 18:17, 2 July 2008

File:1lkd.png

Template:STRUCTURE 1lkd

CRYSTAL STRUCTURE OF 2,3-DIHYDROXYBIPHENYL 1,2-DIOXYGENASE (DHBD) COMPLEXED WITH 2',6'-DICL DIHYDROXYBIPHENYL (DHB)

Template:ABSTRACT PUBMED 12415290

About this Structure

1LKD is a Single protein structure of sequence from Burkholderia sp.. Full crystallographic information is available from OCA.

Reference

Identification and analysis of a bottleneck in PCB biodegradation., Dai S, Vaillancourt FH, Maaroufi H, Drouin NM, Neau DB, Snieckus V, Bolin JT, Eltis LD, Nat Struct Biol. 2002 Dec;9(12):934-9. PMID:12415290

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