8tfg: Difference between revisions

Line 1:

~~'''Unreleased structure'''~~

~~The entry~~ 8tfg is ~~ON HOLD~~

==1.88A CRYSTAL STRUCTURE OF MYCOBACTERIUM TUBERCULOSIS TOPOISOMERASE I==

~~Authors~~: ~~Tan~~, K., ~~Tse~~-~~Dinh~~, Y.C.

== Structural highlights ==

<table><tr><td colspan='2'>[[8tfg]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8TFG OCA]. For a guided tour on the structure components use [https://proteopedia.org/fgij/fg.htm?mol=8TFG FirstGlance].

~~Description~~: 1.~~88A CRYSTAL STRUCTURE OF MYCOBACTERIUM TUBERCULOSIS TOPOISOMERASE I~~

</td></tr><tr id='method'><td class="sblockLbl">[[Empirical_models|Method:]]</td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.88Å</td></tr>

[[Category: ~~Unreleased~~ Structures]]

<tr id='ligand'><td class="sblockLbl">[[Ligand|Ligands:]]</td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr>

[[Category: ~~Tse-Dinh, Y.C~~]]

<tr id='resources'><td class="sblockLbl">Resources:</td><td class="sblockDat">[https://proteopedia.org/fgij/fg.htm?mol=8tfg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8tfg OCA], [https://pdbe.org/8tfg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8tfg RCSB], [https://www.ebi.ac.uk/pdbsum/8tfg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8tfg ProSAT]</td></tr>

[[Category: Tan, K]]

</table>

== Function ==

[https://www.uniprot.org/uniprot/A0A0E8VY41_MYCTX A0A0E8VY41_MYCTX] Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone.[HAMAP-Rule:MF_00952]

__TOC__

</StructureSection>

[[Category: Large Structures]]

[[Category: Mycobacterium tuberculosis]]

[[Category: Tan K]]

[[Category: Tse-Dinh YC]]

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@@ Line 1: / Line 1: @@
-'''Unreleased structure'''
-The entry 8tfg is ON HOLD
+==1.88A CRYSTAL STRUCTURE OF MYCOBACTERIUM TUBERCULOSIS TOPOISOMERASE I==
+<StructureSection load='8tfg' size='340' side='right'caption='[[8tfg]], [[Resolution|resolution]] 1.88&Aring;' scene=''>
-Authors: Tan, K., Tse-Dinh, Y.C.
+== Structural highlights ==
+<table><tr><td colspan='2'>[[8tfg]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8TFG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8TFG FirstGlance]. <br>
-Description: 1.88A CRYSTAL STRUCTURE OF MYCOBACTERIUM TUBERCULOSIS TOPOISOMERASE I
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.88&#8491;</td></tr>
-[[Category: Unreleased Structures]]
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr>
-[[Category: Tse-Dinh, Y.C]]
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8tfg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8tfg OCA], [https://pdbe.org/8tfg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8tfg RCSB], [https://www.ebi.ac.uk/pdbsum/8tfg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8tfg ProSAT]</span></td></tr>
-[[Category: Tan, K]]
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/A0A0E8VY41_MYCTX A0A0E8VY41_MYCTX] Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone.[HAMAP-Rule:MF_00952]
+__TOC__
+</StructureSection>
+[[Category: Large Structures]]
+[[Category: Mycobacterium tuberculosis]]
+[[Category: Tan K]]
+[[Category: Tse-Dinh YC]]