|
|
| Line 1: |
Line 1: |
| [[Image:1llf.gif|left|200px]] | | {{Seed}} |
| | [[Image:1llf.png|left|200px]] |
|
| |
|
| <!-- | | <!-- |
| Line 9: |
Line 10: |
| {{STRUCTURE_1llf| PDB=1llf | SCENE= }} | | {{STRUCTURE_1llf| PDB=1llf | SCENE= }} |
|
| |
|
| '''Cholesterol Esterase (Candida Cylindracea) Crystal Structure at 1.4A resolution'''
| | ===Cholesterol Esterase (Candida Cylindracea) Crystal Structure at 1.4A resolution=== |
|
| |
|
|
| |
|
| ==Overview==
| | <!-- |
| The three-dimensional structure of a Candida cylindracea cholesterol esterase (ChE) homodimer (534 x 2 amino acids) in complex with a ligand of proposed formula C(23)H(48)O(2) has been determined at 1.4 A resolution in space group P1 using synchrotron low-temperature data. The structure refined to R = 0.136 and R(free) = 0.169 and has revealed new stereochemical details in addition to those detected for the apo- and holo-forms at 1.9 and 2.0 A resolution, respectively [Ghosh et al. (1995), Structure, 3, 279-288]. The cholesterol esterase structure is a dimer with four spatially separated interfacial contact areas and two symmetry-related pairs of openings to an internal intradimer cavity. Hydrophobic active-site gorges in each subunit face each other across a central interfacial cavity. The ChE subunits have carbohydrate chains attached to their Asn314 and Asn351 residues, with two ordered N-acetyl-D-glucosoamine moieties visible at each site. The side chains of 14 residues have two alternative conformations with occupancy values of 0.5 +/- 0.2. For each subunit the electron density in the enzyme active-site gorge is well modeled by a C(23)-chain fatty acid.
| | The line below this paragraph, {{ABSTRACT_PUBMED_12499539}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 12499539 is the PubMed ID number. |
| | --> |
| | {{ABSTRACT_PUBMED_12499539}} |
|
| |
|
| ==About this Structure== | | ==About this Structure== |
| Line 30: |
Line 34: |
| [[Category: Crystal structure]] | | [[Category: Crystal structure]] |
| [[Category: Sterol ester acylhydrolase]] | | [[Category: Sterol ester acylhydrolase]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 00:02:06 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul 2 21:22:35 2008'' |
