8k2y: Difference between revisions
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==Crystal structure of MucD== | |||
<StructureSection load='8k2y' size='340' side='right'caption='[[8k2y]], [[Resolution|resolution]] 2.70Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[8k2y]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_syringae_pv._syringae_FF5 Pseudomonas syringae pv. syringae FF5]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8K2Y OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8K2Y FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.7Å</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8k2y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8k2y OCA], [https://pdbe.org/8k2y PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8k2y RCSB], [https://www.ebi.ac.uk/pdbsum/8k2y PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8k2y ProSAT]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Protein quality control mechanisms are essential for maintaining cellular integrity, and the HtrA family of serine proteases plays a crucial role in handling folding stress in prokaryotic periplasm. Escherichia coli harbors three HtrA members, namely, DegS, DegP, and DegQ, which share a common domain structure. MucD, a putative HtrA family member that resembles DegP, is involved in alginate biosynthesis regulation and the stress response. Pseudomonas syringae causes plant diseases and opportunistic infections in humans. This study presents the high-resolution structure of MucD from Pseudomonas syringae (psMucD), revealing its composition as a typical HtrA family serine protease with protease and PDZ domains. Its findings suggest that psMucD containing one PDZ domain is a trimer in solution, and psMucD trimerization is mediated by its N-terminal loop. Sequence and structural analyses revealed similarities and differences with other HtrA family members. Additionally, this study provides a model of psMucD's catalytic process, comparing it with other members of the HtrA family of serine proteases. | |||
The structure of MucD from Pseudomonas syringae revealed N-terminal loop-mediated trimerization of HtrA-like serine protease.,Kim JH, Lee GH, Jeong JH, Kim YG, Park HH Biochem Biophys Res Commun. 2023 Dec 25;688:149175. doi: , 10.1016/j.bbrc.2023.149175. Epub 2023 Nov 4. PMID:37976815<ref>PMID:37976815</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
[[Category: Kim | <div class="pdbe-citations 8k2y" style="background-color:#fffaf0;"></div> | ||
[[Category: Park | == References == | ||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Large Structures]] | |||
[[Category: Pseudomonas syringae pv. syringae FF5]] | |||
[[Category: Kim JH]] | |||
[[Category: Park HH]] | |||
