|
|
| Line 1: |
Line 1: |
| [[Image:1lqb.gif|left|200px]] | | {{Seed}} |
| | [[Image:1lqb.png|left|200px]] |
|
| |
|
| <!-- | | <!-- |
| Line 9: |
Line 10: |
| {{STRUCTURE_1lqb| PDB=1lqb | SCENE= }} | | {{STRUCTURE_1lqb| PDB=1lqb | SCENE= }} |
|
| |
|
| '''Crystal structure of a hydroxylated HIF-1 alpha peptide bound to the pVHL/elongin-C/elongin-B complex'''
| | ===Crystal structure of a hydroxylated HIF-1 alpha peptide bound to the pVHL/elongin-C/elongin-B complex=== |
|
| |
|
|
| |
|
| ==Overview==
| | <!-- |
| Hypoxia-inducible factor-1 (HIF-1) is a transcriptional complex that controls cellular and systemic homeostatic responses to oxygen availability. HIF-1 alpha is the oxygen-regulated subunit of HIF-1, an alpha beta heterodimeric complex. HIF-1 alpha is stable in hypoxia, but in the presence of oxygen it is targeted for proteasomal degradation by the ubiquitination complex pVHL, the protein of the von Hippel Lindau (VHL) tumour suppressor gene and a component of an E3 ubiquitin ligase complex. Capture of HIF-1 alpha by pVHL is regulated by hydroxylation of specific prolyl residues in two functionally independent regions of HIF-1 alpha. The crystal structure of a hydroxylated HIF-1 alpha peptide bound to VCB (pVHL, elongins C and B) and solution binding assays reveal a single, conserved hydroxyproline-binding pocket in pVHL. Optimized hydrogen bonding to the buried hydroxyprolyl group confers precise discrimination between hydroxylated and unmodified prolyl residues. This mechanism provides a new focus for development of therapeutic agents to modulate cellular responses to hypoxia.
| | The line below this paragraph, {{ABSTRACT_PUBMED_12050673}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 12050673 is the PubMed ID number. |
| | --> |
| | {{ABSTRACT_PUBMED_12050673}} |
|
| |
|
| ==About this Structure== | | ==About this Structure== |
| Line 38: |
Line 42: |
| [[Category: Tumor suppressor]] | | [[Category: Tumor suppressor]] |
| [[Category: Ubiquitin]] | | [[Category: Ubiquitin]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 00:10:19 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul 2 21:46:56 2008'' |
