1lwl: Difference between revisions

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[[Image:1lwl.png|left|200px]]

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~~'''~~Crystal Structure of Cytochrome P450-cam with a Fluorescent Probe D-8-Ad (Adamantane-1-carboxylic acid-5-dimethylamino-naphthalene-1-sulfonylamino-octyl-amide)~~'''~~

===Crystal Structure of Cytochrome P450-cam with a Fluorescent Probe D-8-Ad (Adamantane-1-carboxylic acid-5-dimethylamino-naphthalene-1-sulfonylamino-octyl-amide)===

~~==Overview==~~

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~~We have synthesized two luminescent probes (D~~-4-~~Ad and D-8-Ad) that target cytochrome P450cam. D-4-Ad luminescence is quenched by Forster energy transfer upon binding (Kd = 0.83 muM) but is restored when~~ the ~~probe is displaced from~~ the ~~active site by camphor~~. ~~In contrast, D-8-Ad (Kd approximately 0~~.~~02 muM~~) is ~~not displaced from the enzyme, even in~~ the ~~presence of a large excess of camphor~~. ~~The 2.2 A resolution crystal structure of the D~~-8-Ad:P450cam complex reveals extensive hydrophobic contacts between the probe and the enzyme, which result from the conformational flexibility of the B', F, and G helices. Probes with properties similar to those of D-4-Ad potentially could be useful for screening P450 inhibitors.

The line below this paragraph, {{ABSTRACT_PUBMED_12197708}}, adds the Publication Abstract to the page

(as it appears on PubMed at http://www.pubmed.gov), where 12197708 is the PubMed ID number.

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==About this Structure==

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[[Category: Monooxygenase]]

[[Category: Substrate-binding]]

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Sat May~~ ~~3 00~~:22:26 2008''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul 2 22:42:51 2008''

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-[[Image:1lwl.jpg|left|200px]]
+{{Seed}}
+[[Image:1lwl.png|left|200px]]
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 {{STRUCTURE_1lwl|  PDB=1lwl  |  SCENE=  }}
-'''Crystal Structure of Cytochrome P450-cam with a Fluorescent Probe D-8-Ad (Adamantane-1-carboxylic acid-5-dimethylamino-naphthalene-1-sulfonylamino-octyl-amide)'''
+===Crystal Structure of Cytochrome P450-cam with a Fluorescent Probe D-8-Ad (Adamantane-1-carboxylic acid-5-dimethylamino-naphthalene-1-sulfonylamino-octyl-amide)===
-==Overview==
+<!--
-We have synthesized two luminescent probes (D-4-Ad and D-8-Ad) that target cytochrome P450cam. D-4-Ad luminescence is quenched by Forster energy transfer upon binding (Kd = 0.83 muM) but is restored when the probe is displaced from the active site by camphor. In contrast, D-8-Ad (Kd approximately 0.02 muM) is not displaced from the enzyme, even in the presence of a large excess of camphor. The 2.2 A resolution crystal structure of the D-8-Ad:P450cam complex reveals extensive hydrophobic contacts between the probe and the enzyme, which result from the conformational flexibility of the B', F, and G helices. Probes with properties similar to those of D-4-Ad potentially could be useful for screening P450 inhibitors.
+The line below this paragraph, {{ABSTRACT_PUBMED_12197708}}, adds the Publication Abstract to the page
+(as it appears on PubMed at http://www.pubmed.gov), where 12197708 is the PubMed ID number.
+-->
+{{ABSTRACT_PUBMED_12197708}}
 ==About this Structure==
@@ Line 37: / Line 41: @@
 [[Category: Monooxygenase]]
 [[Category: Substrate-binding]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 00:22:26 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul  2 22:42:51 2008''