1lwt: Difference between revisions

From Proteopedia
Jump to navigationJump to search
← Older editNewer edit →
OCA (talk | contribs)
Bots, Bureaucrats, journal, Administrators
3,329,557 edits
No edit summary
OCA (talk | contribs)
Bots, Bureaucrats, journal, Administrators
3,329,557 edits
No edit summary
Line 1: Line 1:
[[Image:1lwt.gif|left|200px]]
{{Seed}}
[[Image:1lwt.png|left|200px]]


<!--
<!--
Line 9: Line 10:
{{STRUCTURE_1lwt|  PDB=1lwt  |  SCENE=  }}  
{{STRUCTURE_1lwt|  PDB=1lwt  |  SCENE=  }}  


'''Crystal structure of the intein homing endonuclease PI-SceI bound to its substrate DNA (Ca2+ free)'''
===Crystal structure of the intein homing endonuclease PI-SceI bound to its substrate DNA (Ca2+ free)===




==Overview==
<!--
The first X-ray structures of an intein-DNA complex, that of the two-domain homing endonuclease PI-SceI bound to its 36-base pair DNA substrate, have been determined in the presence and absence of Ca(2+). The DNA shows an asymmetric bending pattern, with a major 50 degree bend in the endonuclease domain and a minor 22 degree bend in the splicing domain region. Distortions of the DNA bound to the endonuclease domain cause the insertion of the two cleavage sites in the catalytic center. DNA binding induces changes in the protein conformation. The two overlapping non-identical active sites in the endonucleolytic center contain two Ca(+2) ions that coordinate to the catalytic Asp residues. Structure analysis indicates that the top strand may be cleaved first.
The line below this paragraph, {{ABSTRACT_PUBMED_12219083}}, adds the Publication Abstract to the page
(as it appears on PubMed at http://www.pubmed.gov), where 12219083 is the PubMed ID number.
-->
{{ABSTRACT_PUBMED_12219083}}


==About this Structure==
==About this Structure==
Line 29: Line 33:
[[Category: Intein]]
[[Category: Intein]]
[[Category: Protein-dna complex]]
[[Category: Protein-dna complex]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 00:22:48 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul 2 22:43:41 2008''
Retrieved from "https://proteopedia.org/1lwt"