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| {{STRUCTURE_1ly2| PDB=1ly2 | SCENE= }} | | {{STRUCTURE_1ly2| PDB=1ly2 | SCENE= }} |
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| '''Crystal structure of unliganded human CD21 SCR1-SCR2 (Complement receptor type 2)'''
| | ===Crystal structure of unliganded human CD21 SCR1-SCR2 (Complement receptor type 2)=== |
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| ==Overview==
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| Human complement receptor type 2 (CD21) is the cellular receptor for Epstein-Barr virus (EBV), a human tumor virus. The N-terminal two short consensus repeats (SCR1-SCR2) of the receptor interact with the EBV glycoprotein gp350/220 and also with the natural CD21 ligand C3d. Here we present the crystal structure of the CD21 SCR1-SCR2 fragment in the absence of ligand and demonstrate that it is able to bind EBV. Based on a functional analysis of wild-type and mutant CD21 and molecular modeling, we identify a likely region for EBV attachment and demonstrate that this region is not involved in the interaction with C3d. A comparison with the previously determined structure of CD21 SCR1-SCR2 in complex with C3d shows that, in both cases, CD21 assumes compact V-shaped conformations. However, our analysis reveals a surprising degree of flexibility at the SCR1-SCR2 interface, suggesting interactions between the two domains are not specific. We present evidence that the V-shaped conformation is induced by deglycosylation of the protein, and that physiologic glycosylation of CD21 would result in a more extended conformation, perhaps with additional epitopes for C3d binding.
| | The line below this paragraph, {{ABSTRACT_PUBMED_12122212}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 12122212 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_12122212}} |
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| ==Disease== | | ==Disease== |
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| [[Category: Short consensus repeat]] | | [[Category: Short consensus repeat]] |
| [[Category: Viral receptor]] | | [[Category: Viral receptor]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 00:24:42 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul 2 22:47:50 2008'' |
