1lwv: Difference between revisions
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New page: left|200px<br /> <applet load="1lwv" size="450" color="white" frame="true" align="right" spinBox="true" caption="1lwv, resolution 2.3Å" /> '''Borohydride-trapped ... |
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[[Image:1lwv.gif|left|200px]]<br /> | [[Image:1lwv.gif|left|200px]]<br /><applet load="1lwv" size="350" color="white" frame="true" align="right" spinBox="true" | ||
<applet load="1lwv" size=" | |||
caption="1lwv, resolution 2.3Å" /> | caption="1lwv, resolution 2.3Å" /> | ||
'''Borohydride-trapped hOgg1 Intermediate Structure Co-Crystallized with 8-aminoguanine'''<br /> | '''Borohydride-trapped hOgg1 Intermediate Structure Co-Crystallized with 8-aminoguanine'''<br /> | ||
==Overview== | ==Overview== | ||
Most spontaneous damage to bases in DNA is corrected through the action of | Most spontaneous damage to bases in DNA is corrected through the action of the base-excision DNA repair pathway. Base excision repair is initiated by DNA glycosylases, lesion-specific enzymes that intercept aberrant bases in DNA and catalyze their excision. How such proteins accomplish the feat of catalyzing no fewer than five sequential reaction steps using a single active site has been unknown. To help answer this, we report the structure of a trapped catalytic intermediate in DNA repair by human 8-oxoguanine DNA glycosylase. This structure and supporting biochemical results reveal that the enzyme sequesters the excised lesion base and exploits it as a cofactor to participate in catalysis. To our knowledge, the present example represents the first documented case of product-assisted catalysis in an enzyme-catalyzed reaction. | ||
==Disease== | ==Disease== | ||
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==About this Structure== | ==About this Structure== | ||
1LWV is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with CA and ANG as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | 1LWV is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=CA:'>CA</scene> and <scene name='pdbligand=ANG:'>ANG</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LWV OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Bruner, S | [[Category: Bruner, S D.]] | ||
[[Category: Fromme, J | [[Category: Fromme, J C.]] | ||
[[Category: Karplus, M.]] | [[Category: Karplus, M.]] | ||
[[Category: Verdine, G | [[Category: Verdine, G L.]] | ||
[[Category: Yang, W.]] | [[Category: Yang, W.]] | ||
[[Category: ANG]] | [[Category: ANG]] | ||
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[[Category: reaction intermediate]] | [[Category: reaction intermediate]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:49:17 2008'' | ||
