1m2n: Difference between revisions

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[[Image:1m2n.jpg|left|200px]]
{{Seed}}
[[Image:1m2n.png|left|200px]]


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{{STRUCTURE_1m2n|  PDB=1m2n  |  SCENE=  }}  
{{STRUCTURE_1m2n|  PDB=1m2n  |  SCENE=  }}  


'''Sir2 homologues (D102G/F159A/R170A) mutant-2'-O-acetyl ADP ribose complex'''
===Sir2 homologues (D102G/F159A/R170A) mutant-2'-O-acetyl ADP ribose complex===




==Overview==
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The NAD-dependent histone/protein deacetylase activity of Sir2 (silent information regulator 2) accounts for its diverse biological roles including gene silencing, DNA damage repair, cell cycle regulation, and life span extension. We provide crystallographic evidence that 2'-O-acetyl ADP-ribose is the reaction product that is formed at the active site of Sir2 from the 2.6-A co-crystal structure of 2'-O-acetyl-ADP-ribose and Sir2 from Archaeoglobus fulgidus. In addition, we show that His-116 and Phe-159 play critical roles in the catalysis and substrate recognition. The conserved Ser-24 and Asp-101 contribute to the stability for NAD binding rather than being directly involved in the catalysis. The crystal structures of wild type and mutant derivatives of Sir2, in conjunction with biochemical analyses of the mutants, provide novel insights into the reaction mechanism of Sir2-mediated deacetylation.
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==About this Structure==
==About this Structure==
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[[Category: Cho, Y.]]
[[Category: Cho, Y.]]
[[Category: Protein-ligand complex]]
[[Category: Protein-ligand complex]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 00:33:34 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul 2 23:05:06 2008''

Revision as of 20:05, 2 July 2008

File:1m2n.png

Template:STRUCTURE 1m2n

Sir2 homologues (D102G/F159A/R170A) mutant-2'-O-acetyl ADP ribose complex

Template:ABSTRACT PUBMED 12091395

About this Structure

1M2N is a Single protein structure of sequence from Archaeoglobus fulgidus. Full crystallographic information is available from OCA.

Reference

Structural basis for the NAD-dependent deacetylase mechanism of Sir2., Chang JH, Kim HC, Hwang KY, Lee JW, Jackson SP, Bell SD, Cho Y, J Biol Chem. 2002 Sep 13;277(37):34489-98. Epub 2002 Jun 28. PMID:12091395

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