1m39: Difference between revisions

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[[Image:1m39.gif|left|200px]]
{{Seed}}
[[Image:1m39.png|left|200px]]


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{{STRUCTURE_1m39|  PDB=1m39  |  SCENE=  }}  
{{STRUCTURE_1m39|  PDB=1m39  |  SCENE=  }}  


'''Solution structure of the C-terminal fragment (F86-I165) of the human centrin 2 in calcium saturated form'''
===Solution structure of the C-terminal fragment (F86-I165) of the human centrin 2 in calcium saturated form===




==Overview==
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Human centrin 2 (HsCen2) is an EF-hand protein that plays a critical role in the centrosome duplication and separation during cell division. We studied the structural and Ca(2+)-binding properties of two C-terminal fragments of this protein: SC-HsCen2 (T94-Y172), covering two EF-hands, and LC-HsCen2 (M84-Y172), having 10 additional residues. Both fragments are highly disordered in the apo state but become better structured (although not conformationally homogeneous) in the presence of Ca(2+) and depending on the nature of the cations (K(+) or Na(+)) in the buffer. Only the longer C-terminal domain, in the Ca(2+)-saturated state and in the presence of Na(+) ions, was amenable to structure determination by nuclear magnetic resonance. The solution structure of LC-HsCen2 reveals an open two EF-hand structure, similar to the conformation of related Ca(2+)-saturated regulatory domains. Unexpectedly, the N-terminal helix segment (F86-T94) lies over the exposed hydrophobic cavity. This unusual intramolecular interaction increases considerably the Ca(2+) affinity and constitutes a useful model for the target binding.
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{{ABSTRACT_PUBMED_12578356}}


==About this Structure==
==About this Structure==
1M39 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1M39 OCA].  
1M39 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1M39 OCA].  


==Reference==
==Reference==
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[[Category: Miron, S.]]
[[Category: Miron, S.]]
[[Category: Ef-hand]]
[[Category: Ef-hand]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 00:34:50 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul 2 23:07:26 2008''

Revision as of 20:07, 2 July 2008

File:1m39.png

Template:STRUCTURE 1m39

Solution structure of the C-terminal fragment (F86-I165) of the human centrin 2 in calcium saturated form

Template:ABSTRACT PUBMED 12578356

About this Structure

1M39 is a Single protein structure of sequence from Homo sapiens. Full experimental information is available from OCA.

Reference

C-terminal half of human centrin 2 behaves like a regulatory EF-hand domain., Matei E, Miron S, Blouquit Y, Duchambon P, Durussel I, Cox JA, Craescu CT, Biochemistry. 2003 Feb 18;42(6):1439-50. PMID:12578356

Page seeded by OCA on Wed Jul 2 23:07:26 2008

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