1m4l: Difference between revisions

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[[Image:1m4l.gif|left|200px]]
{{Seed}}
[[Image:1m4l.png|left|200px]]


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{{STRUCTURE_1m4l|  PDB=1m4l  |  SCENE=  }}  
{{STRUCTURE_1m4l|  PDB=1m4l  |  SCENE=  }}  


'''STRUCTURE OF NATIVE CARBOXYPEPTIDASE A AT 1.25 RESOLUTION'''
===STRUCTURE OF NATIVE CARBOXYPEPTIDASE A AT 1.25 RESOLUTION===




==Overview==
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The crystal structure of the bovine zinc metalloproteinase carboxypeptidase A (CPA) has been refined to 1.25 A resolution based on room-temperature X-ray synchrotron data. The significantly improved structure of CPA at this resolution (anisotropic temperature factors, R factor = 10.4%, R(free) = 14.5%) allowed the modelling of conformational disorders of side chains, improved the description of the protein solvent network (375 water molecules) and provided a more accurate picture of the interactions between the active-site zinc and its ligands. The calculation of standard uncertainties in individual atom positions of the refined model of CPA allowed the deduction of the protonation state of some key residues in the active site and confirmed that Glu72 and Glu270 are negatively charged in the resting state of the enzyme at pH 7.5. These results were further validated by theoretical calculations that showed significant reduction of the pK(a) of these side chains relative to solution values. The distance between the zinc-bound solvent molecule and the metal ion is strongly suggestive of a neutral water molecule and not a hydroxide ion in the resting state of the enzyme. These findings could support both the general acid/general base mechanism, as well as the anhydride mechanism suggested for CPA.
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{{ABSTRACT_PUBMED_12554943}}


==About this Structure==
==About this Structure==
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[[Category: Metalloexoproteinase]]
[[Category: Metalloexoproteinase]]
[[Category: Metalloproteinase]]
[[Category: Metalloproteinase]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 00:37:19 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul 2 23:11:37 2008''

Revision as of 20:11, 2 July 2008

File:1m4l.png

Template:STRUCTURE 1m4l

STRUCTURE OF NATIVE CARBOXYPEPTIDASE A AT 1.25 RESOLUTION

Template:ABSTRACT PUBMED 12554943

About this Structure

1M4L is a Single protein structure of sequence from Bos taurus. Full crystallographic information is available from OCA.

Reference

Refined structure of bovine carboxypeptidase A at 1.25 A resolution., Kilshtain-Vardi A, Glick M, Greenblatt HM, Goldblum A, Shoham G, Acta Crystallogr D Biol Crystallogr. 2003 Feb;59(Pt 2):323-33. Epub 2003, Jan 23. PMID:12554943

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