5hy0

orotic acid hydrolase

spinqualitylabelsall models 5hy0, resolution 2.40Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 5hy0 is a 4 chain structure with sequence from Frankia sp. Eul1b. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2.4Å Ligands: GOL Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function CYAH_PSEI1 Cyclic amide hydrolase of unknown substrate specificity. Catalyzes the hydrolytic ring-opening of a cyclic amide. Does not act on cyanuric acid nor barbituric acid.[HAMAP-Rule:MF_01989] Publication Abstract from PubMed The Toblerone-fold was discovered recently when the first structure of the cyclic amide hydrolase, AtzD (a cyanuric acid hydrolase), was elucidated. We surveyed the cyclic amide hydrolase family finding a strong correlation between phylogenetic distribution and specificity for either cyanuric acid or barbituric acid; albeit, one of six classes (IV) could not be tested due to lack of expression of the proteins from it, and another class (V) had neither cyanuric acid nor barbituric acid hydrolase activity. High resolution X-ray structures were obtained for a class VI barbituric acid hydrolase (1.7 A) from a Rhodococcus sp. and a class V cyclic amide hydrolase (2.4 A) from a Frankia sp. for which we were unable to identify a substrate. Both structures were homologous with the tetrameric Toblerone-fold enzyme AtzD, demonstrating a high degree of structural conservation within the cyclic amide hydrolase family. The barbituric acid hydrolase structure did not contain zinc, in contrast with early reports of zinc-dependent activity for this enzyme. Instead, each barbituric acid hydrolase monomer contained either a Na+ or a Mg2+, analogous to the structural metal found in cyanuric acid hydrolase. The Frankia cyclic amide hydrolase contained no metal, but instead formed unusual, reversible, intermolecular vicinal disulfide bonds that contributed to the thermal stability of the protein. The active sites were largely conserved between the three enzymes, differing at six positions, which likely determine substrate specificity.Importance The 'Toblerone-fold' enzymes catalyze an unusual ring-opening hydrolysis with cyclic amide substrates. A survey of these enzymes shows that there is a good correlation between physiological function and phylogenetic distribution within this family of enzymes, and provide insights into the evolutionary relationships between the cyanuric acid and barbituric acid hydrolases.This family of enzymes is structurally and mechanistically distinct from other enzyme families; however, to date the structure of just two, physiologically identical, enzymes from this family have been described. We present two new structures: a barbituric acid hydrolase and an enzyme of unknown function. These structures confirm that the CyAH family share the unusual Toblerone fold, albeit with some significant differences. High resolution X-ray structures of two functionally distinct members of the cyclic amide hydrolase (CyAH) family of Toblerone fold enzymes.,Peat TS, Balotra S, Wilding M, Hartley CJ, Newman J, Scott C Appl Environ Microbiol. 2017 Feb 24. pii: AEM.03365-16. doi:, 10.1128/AEM.03365-16. PMID:28235873 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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