1m8m: Difference between revisions

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[[Image:1m8m.jpg|left|200px]]
{{Seed}}
[[Image:1m8m.png|left|200px]]


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{{STRUCTURE_1m8m|  PDB=1m8m  |  SCENE=  }}  
{{STRUCTURE_1m8m|  PDB=1m8m  |  SCENE=  }}  


'''SOLID-STATE MAS NMR STRUCTURE OF THE A-SPECTRIN SH3 DOMAIN'''
===SOLID-STATE MAS NMR STRUCTURE OF THE A-SPECTRIN SH3 DOMAIN===




==Overview==
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The determination of a representative set of protein structures is a chief aim in structural genomics. Solid-state NMR may have a crucial role in structural investigations of those proteins that do not easily form crystals or are not accessible to solution NMR, such as amyloid systems or membrane proteins. Here we present a protein structure determined by solid-state magic-angle-spinning (MAS) NMR. Almost complete (13)C and (15)N resonance assignments for a micro-crystalline preparation of the alpha-spectrin Src-homology 3 (SH3) domain formed the basis for the extraction of a set of distance restraints. These restraints were derived from proton-driven spin diffusion (PDSD) spectra of biosynthetically site-directed, labelled samples obtained from bacteria grown using [1,3-(13)C]glycerol or [2-(13)C]glycerol as carbon sources. This allowed the observation of long-range distance correlations up to approximately 7 A. The calculated global fold of the alpha-spectrin SH3 domain is based on 286 inter-residue (13)C-(13)C and six (15)N-(15)N restraints, all self-consistently obtained by solid-state MAS NMR. This MAS NMR procedure should be widely applicable to small membrane proteins that can be expressed in bacteria.
The line below this paragraph, {{ABSTRACT_PUBMED_12422222}}, adds the Publication Abstract to the page
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{{ABSTRACT_PUBMED_12422222}}


==About this Structure==
==About this Structure==
1M8M is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1M8M OCA].  
1M8M is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1M8M OCA].  


==Reference==
==Reference==
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[[Category: Schubert, M.]]
[[Category: Schubert, M.]]
[[Category: Solid-state mas nmr structure]]
[[Category: Solid-state mas nmr structure]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 00:45:53 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul 2 23:26:48 2008''

Revision as of 20:26, 2 July 2008

File:1m8m.png

Template:STRUCTURE 1m8m

SOLID-STATE MAS NMR STRUCTURE OF THE A-SPECTRIN SH3 DOMAIN

Template:ABSTRACT PUBMED 12422222

About this Structure

1M8M is a Single protein structure of sequence from Gallus gallus. Full experimental information is available from OCA.

Reference

Structure of a protein determined by solid-state magic-angle-spinning NMR spectroscopy., Castellani F, van Rossum B, Diehl A, Schubert M, Rehbein K, Oschkinat H, Nature. 2002 Nov 7;420(6911):98-102. PMID:12422222

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