1m8n: Difference between revisions

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[[Image:1m8n.gif|left|200px]]
{{Seed}}
[[Image:1m8n.png|left|200px]]


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{{STRUCTURE_1m8n|  PDB=1m8n  |  SCENE=  }}  
{{STRUCTURE_1m8n|  PDB=1m8n  |  SCENE=  }}  


'''Choristoneura Fumiferana (Spruce Budworm) Antifreeze Protein Isoform 501'''
===Choristoneura Fumiferana (Spruce Budworm) Antifreeze Protein Isoform 501===




==Overview==
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The insect spruce budworm (Choristoneura fumiferana)(Cf) produces a number of isoforms of its highly active antifreeze protein (CfAFP). Although most of the CfAFP isoforms are in the 9-kDa range, isoforms containing a 30- or 31-amino acid insertion have also been identified. Here we describe the functional and structural analysis of a selected long isoform, CfAFP-501. X-ray crystal structure determination reveals that the 31-amino acid insertion found in CfAFP-501 forms two additional loops within its highly regular beta-helical structure. This effectively extends the area of the two-dimensional Thr array and ice-binding surface of the protein. The larger isoform has 3 times the thermal hysteresis activity of the 9-kDa CfAFP-337. As well, a deletion of the 31-amino acid insertion within CfAFP-501 to form CfAFP-501-Delta-2-loop, results in a protein with reduced activity similar to the shorter CfAFP isoforms. Thus, the enhanced antifreeze activity of CfAFP-501 is directly correlated to the length of its beta-helical structure and hence the size of its ice-binding face.
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{{ABSTRACT_PUBMED_12105229}}


==About this Structure==
==About this Structure==
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[[Category: Antifreeze protein]]
[[Category: Antifreeze protein]]
[[Category: Left-handed beta-helix]]
[[Category: Left-handed beta-helix]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 00:46:02 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul 2 23:26:55 2008''

Revision as of 20:26, 2 July 2008

File:1m8n.png

Template:STRUCTURE 1m8n

Choristoneura Fumiferana (Spruce Budworm) Antifreeze Protein Isoform 501

Template:ABSTRACT PUBMED 12105229

About this Structure

1M8N is a Single protein structure of sequence from Choristoneura fumiferana. Full crystallographic information is available from OCA.

Reference

A beta-helical antifreeze protein isoform with increased activity. Structural and functional insights., Leinala EK, Davies PL, Doucet D, Tyshenko MG, Walker VK, Jia Z, J Biol Chem. 2002 Sep 6;277(36):33349-52. Epub 2002 Jun 24. PMID:12105229

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