1mb8: Difference between revisions
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New page: left|200px<br /> <applet load="1mb8" size="450" color="white" frame="true" align="right" spinBox="true" caption="1mb8, resolution 2.15Å" /> '''Crystal Structure o... |
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[[Image:1mb8.gif|left|200px]]<br /> | [[Image:1mb8.gif|left|200px]]<br /><applet load="1mb8" size="350" color="white" frame="true" align="right" spinBox="true" | ||
<applet load="1mb8" size=" | |||
caption="1mb8, resolution 2.15Å" /> | caption="1mb8, resolution 2.15Å" /> | ||
'''Crystal Structure of the actin binding domain of plectin'''<br /> | '''Crystal Structure of the actin binding domain of plectin'''<br /> | ||
==Overview== | ==Overview== | ||
Plectin is a widely expressed cytoskeletal linker. Here we report the | Plectin is a widely expressed cytoskeletal linker. Here we report the crystal structure of the actin binding domain of plectin and show that this region is sufficient for interaction with F-actin or the cytoplasmic region of integrin alpha6beta4. The structure is formed by two calponin homology domains arranged in a closed conformation. We show that binding to F-actin induces a conformational change in plectin that is inhibited by an engineered interdomain disulfide bridge. A two-step induced fit mechanism involving binding and subsequent domain rearrangement is proposed. In contrast, interaction with integrin alpha6beta4 occurs in a closed conformation. Competitive binding of plectin to F-actin and integrin alpha6beta4 may rely on the observed alternative binding mechanisms and involve both allosteric and steric factors. | ||
==Disease== | ==Disease== | ||
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==About this Structure== | ==About this Structure== | ||
1MB8 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http:// | 1MB8 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MB8 OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Pereda, J | [[Category: Pereda, J M.de.]] | ||
[[Category: actin binding domain]] | [[Category: actin binding domain]] | ||
[[Category: calponin homology domain]] | [[Category: calponin homology domain]] | ||
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[[Category: integrin beta4 hemidesmosomes]] | [[Category: integrin beta4 hemidesmosomes]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:53:26 2008'' | ||
