1mf8: Difference between revisions
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New page: left|200px<br /> <applet load="1mf8" size="450" color="white" frame="true" align="right" spinBox="true" caption="1mf8, resolution 3.1Å" /> '''Crystal Structure of... |
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[[Image:1mf8.gif|left|200px]]<br /> | [[Image:1mf8.gif|left|200px]]<br /><applet load="1mf8" size="350" color="white" frame="true" align="right" spinBox="true" | ||
<applet load="1mf8" size=" | |||
caption="1mf8, resolution 3.1Å" /> | caption="1mf8, resolution 3.1Å" /> | ||
'''Crystal Structure of human calcineurin complexed with cyclosporin A and human cyclophilin'''<br /> | '''Crystal Structure of human calcineurin complexed with cyclosporin A and human cyclophilin'''<br /> | ||
==Overview== | ==Overview== | ||
Calcineurin (Cn), a Ca(2+)/calmodulin-dependent Ser/Thr protein | Calcineurin (Cn), a Ca(2+)/calmodulin-dependent Ser/Thr protein phosphatase, is an important participant in signaling pathways that activate T cells. It is the target of the immunosuppressive drugs cyclosporin A (CsA) and FK506. These drugs bind proteins known as cyclophilin (Cyp) and FK506-binding protein, respectively, and the drug-protein complexes in turn inhibit Cn. We report the crystal structure of a Cyp/CsA/Cn ternary complex, determined to a resolution of 3.1 A. Residues 3-9 of CsA, particularly N-methyl leucines 4 and 6, and Trp-121 of Cyp form a composite surface for interaction with Cn. The hydrophobic interface buries two hydrogen bonds. The structure accounts clearly for the effects of mutations in Cn on CsA-resistance and for the way modifications of CsA alter immunosuppressive activity. | ||
==Disease== | ==Disease== | ||
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==About this Structure== | ==About this Structure== | ||
1MF8 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with CA and PO4 as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Phosphoprotein_phosphatase Phosphoprotein phosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.16 3.1.3.16] Full crystallographic information is available from [http:// | 1MF8 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=CA:'>CA</scene> and <scene name='pdbligand=PO4:'>PO4</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Phosphoprotein_phosphatase Phosphoprotein phosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.16 3.1.3.16] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MF8 OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: Phosphoprotein phosphatase]] | [[Category: Phosphoprotein phosphatase]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Harrison, S | [[Category: Harrison, S C.]] | ||
[[Category: Jin, L.]] | [[Category: Jin, L.]] | ||
[[Category: CA]] | [[Category: CA]] | ||
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[[Category: protein-drug complex]] | [[Category: protein-drug complex]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:54:40 2008'' | ||
