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| {{STRUCTURE_1md0| PDB=1md0 | SCENE= }} | | {{STRUCTURE_1md0| PDB=1md0 | SCENE= }} |
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| '''CRYSTAL STRUCTURE OF AN INHIBITED FRAGMENT OF Ets-1'''
| | ===CRYSTAL STRUCTURE OF AN INHIBITED FRAGMENT OF Ets-1=== |
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| ==Overview==
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| The DNA-binding activity of the eukaryotic transcription factor Ets-1 (E26 avian erythroblastosis virus oncogene-E twenty-six) is negatively regulated by inhibitory regions that flank the ETS domain. Based on the results of solution studies, these N- and C-terminal inhibitory regions have been proposed to pack against the ETS domain and form an autoinhibitory module whose N terminus partially unfolds upon binding of Ets-1 to DNA. Mutations that disrupt autoinhibition of DNA binding also cause a structural change in the inhibitory region. We report here a crystallographic study of fragments of Ets-1 that provide structural details of the inhibitory module and the structural transition that accompanies DNA binding. The structures of free and DNA-bound Ets-1 fragments containing the ETS domain and the inhibitory regions confirm that the N-terminal inhibitory region contains two alpha-helices one of which unfolds upon Ets-1 binding to DNA. The observations from the crystal structure, coupled with mutagenesis experiments, allow us to propose a model for the inhibited form of Ets-1 and lend insight into the flexible interaction between Ets-1 and the acute myeloid leukemia 1 protein, AML1 (RUNX1). | | The line below this paragraph, {{ABSTRACT_PUBMED_12221090}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 12221090 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_12221090}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: Autoinhibition]] | | [[Category: Autoinhibition]] |
| [[Category: Transcription factor]] | | [[Category: Transcription factor]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 00:53:58 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul 2 23:42:30 2008'' |
