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| [[Image:1mf1.jpg|left|200px]] | | {{Seed}} |
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| {{STRUCTURE_1mf1| PDB=1mf1 | SCENE= }} | | {{STRUCTURE_1mf1| PDB=1mf1 | SCENE= }} |
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| '''Structure of the Recombinant Mouse-Muscle Adenylosuccinate Synthetase Complexed with AMP'''
| | ===Structure of the Recombinant Mouse-Muscle Adenylosuccinate Synthetase Complexed with AMP=== |
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| ==Overview==
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| Adenylosuccinate synthetase governs the committed step of AMP biosynthesis, the generation of 6-phosphoryl-IMP from GTP and IMP followed by the formation of adenylosuccinate from 6-phosphoryl-IMP and l-aspartate. The enzyme is subject to feedback inhibition by AMP and adenylosuccinate, but crystallographic complexes of the mouse muscle synthetase presented here infer mechanisms of inhibition that involve potentially synergistic ligand combinations. AMP alone adopts the productive binding mode of IMP and yet stabilizes the active site in a conformation that favors the binding of Mg(2+)-IMP to the GTP pocket. On the other hand, AMP, in the presence of GDP, orthophosphate, and Mg(2+), adopts the binding mode of adenylosuccinate. Depending on circumstances then, AMP behaves as an analogue of IMP or as an analogue of adenylosuccinate. The complex of adenylosuccinate.GDP.Mg(2+).sulfate, the first structure of an adenylosuccinate-bound synthetase, reveals significant geometric distortions and tight nonbonded contacts relevant to the proposed catalytic mechanism. Adenylosuccinate forms from 6-phosphoryl-IMP and l-aspartate by the movement of the purine ring into the alpha-amino group of l-aspartate.
| | The line below this paragraph, {{ABSTRACT_PUBMED_12186864}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 12186864 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_12186864}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: Multigene family]] | | [[Category: Multigene family]] |
| [[Category: Purine biosynthesis]] | | [[Category: Purine biosynthesis]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 00:57:30 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul 2 23:49:36 2008'' |
