1mnt: Difference between revisions

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[[Image:1mnt.gif|left|200px]]
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[[Image:1mnt.png|left|200px]]


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{{STRUCTURE_1mnt|  PDB=1mnt  |  SCENE=  }}  
{{STRUCTURE_1mnt|  PDB=1mnt  |  SCENE=  }}  


'''SOLUTION STRUCTURE OF DIMERIC MNT REPRESSOR (1-76)'''
===SOLUTION STRUCTURE OF DIMERIC MNT REPRESSOR (1-76)===




==Overview==
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Wild-type Mnt repressor of Salmonella bacteriophage P22 is a tetrameric protein of 82 residues per monomer. A C-terminal deletion mutant of the repressor denoted Mnt (1-76) is a dimer in solution. The structure of this dimer has been determined using NMR. The NMR assignments of the majority of the 1H, 15N, and 13C resonances were obtained using 2D and triple-resonance 3D techniques. Elements of secondary structure were identified on the basis of characteristic sequential and medium range NOEs. For the structure determination more than 1000 NOEs per monomer were obtained, and structures were generated using distance geometry and restrained simulated annealing calculations. The discrimination of intra- vs intermonomer NOEs was based upon the observation of intersubunit NOEs in [15N,13C] double half-filtered NOESY experiments. The N-terminal part of Mnt (residues 1-44), which shows a 40% sequence homology with the Arc repressor, has a similar secondary and tertiary structure. Mnt (1-76) continues with a loop region of irregular structure, a third alpha-helix, and a random coil C-terminal peptide. Analysis of the secondary structure NOEs, the exchange rates, and the backbone chemical shifts suggests that the carboxy-terminal third helix is less stable than the remainder of the protein, but the observation of intersubunit NOEs for this part of the protein enables the positioning of this helix. The rsmd's between the backbone atoms of the N-terminal part of the Mnt repressor (residues 5-43, 5'-43') and the Arc repressor is 1.58 A, and between this region and the corresponding part of the MetJ repressor 1.43 A.
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{{ABSTRACT_PUBMED_7999761}}


==About this Structure==
==About this Structure==
1MNT is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_p22 Enterobacteria phage p22]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MNT OCA].  
1MNT is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_p22 Enterobacteria phage p22]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MNT OCA].  


==Reference==
==Reference==
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[[Category: Sauer, R T.]]
[[Category: Sauer, R T.]]
[[Category: Transcription regulation]]
[[Category: Transcription regulation]]
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Revision as of 21:29, 2 July 2008

File:1mnt.png

Template:STRUCTURE 1mnt

SOLUTION STRUCTURE OF DIMERIC MNT REPRESSOR (1-76)

Template:ABSTRACT PUBMED 7999761

About this Structure

1MNT is a Single protein structure of sequence from Enterobacteria phage p22. Full experimental information is available from OCA.

Reference

Solution structure of dimeric Mnt repressor (1-76)., Burgering MJ, Boelens R, Gilbert DE, Breg JN, Knight KL, Sauer RT, Kaptein R, Biochemistry. 1994 Dec 20;33(50):15036-45. PMID:7999761

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