1mow: Difference between revisions

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[[Image:1mow.~~gif~~|left|200px]]

[[Image:1mow.png|left|200px]]

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~~'''~~E-DreI~~'''~~

===E-DreI===

~~==Overview==~~

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~~We have generated an artificial highly specific endonuclease by fusing domains of homing endonucleases I~~-~~DmoI and I~~-~~CreI and creating a new 1400 A(2) protein interface between these domains. Protein engineering was accomplished by combining computational redesign and an in vivo protein-folding screen.~~ The ~~resulting enzyme~~, ~~E-DreI~~ (~~Engineered I-DmoI~~/~~I-CreI~~), binds a long chimeric DNA target site with nanomolar affinity, cleaving it precisely at a rate equivalent to its natural parents. The structure of an E-DreI/DNA complex demonstrates the accuracy of the protein interface redesign algorithm and reveals how catalytic function is ~~maintained during~~ the ~~creation of the new endonuclease. These results indicate that it may be possible to generate novel highly specific DNA binding proteins from homing endonucleases~~.

The line below this paragraph, {{ABSTRACT_PUBMED_12419232}}, adds the Publication Abstract to the page

(as it appears on PubMed at http://www.pubmed.gov), where 12419232 is the PubMed ID number.

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==About this Structure==

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[[Category: Laglidadg]]

[[Category: Structure]]

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Sat May~~ 3 01:32:10 2008''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Jul 3 00:36:52 2008''

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-[[Image:1mow.gif|left|200px]]
+{{Seed}}
+[[Image:1mow.png|left|200px]]
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 {{STRUCTURE_1mow|  PDB=1mow  |  SCENE=  }}
-'''E-DreI'''
+===E-DreI===
-==Overview==
+<!--
-We have generated an artificial highly specific endonuclease by fusing domains of homing endonucleases I-DmoI and I-CreI and creating a new 1400 A(2) protein interface between these domains. Protein engineering was accomplished by combining computational redesign and an in vivo protein-folding screen. The resulting enzyme, E-DreI (Engineered I-DmoI/I-CreI), binds a long chimeric DNA target site with nanomolar affinity, cleaving it precisely at a rate equivalent to its natural parents. The structure of an E-DreI/DNA complex demonstrates the accuracy of the protein interface redesign algorithm and reveals how catalytic function is maintained during the creation of the new endonuclease. These results indicate that it may be possible to generate novel highly specific DNA binding proteins from homing endonucleases.
+The line below this paragraph, {{ABSTRACT_PUBMED_12419232}}, adds the Publication Abstract to the page
+(as it appears on PubMed at http://www.pubmed.gov), where 12419232 is the PubMed ID number.
+-->
+{{ABSTRACT_PUBMED_12419232}}
 ==About this Structure==
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 [[Category: Laglidadg]]
 [[Category: Structure]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 01:32:10 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Jul  3 00:36:52 2008''