1mpx: Difference between revisions

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[[Image:1mpx.~~gif~~|left|200px]]

[[Image:1mpx.png|left|200px]]

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~~'''~~ALPHA-AMINO ACID ESTER HYDROLASE LABELED WITH SELENOMETHIONINE~~'''~~

===ALPHA-AMINO ACID ESTER HYDROLASE LABELED WITH SELENOMETHIONINE===

~~==Overview==~~

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~~alpha~~-~~Amino acid ester hydrolases (AEHs) catalyze the hydrolysis and synthesis of esters and amides with an alpha~~-amino group. As such, they can synthesize beta-lactam antibiotics from acyl compounds and beta-lactam nuclei obtained from the hydrolysis of natural antibiotics. This article describes the gene sequence and the 1.9-A resolution crystal structure of the AEH from Xanthomonas citri. The ~~enzyme consists of an alpha/beta-hydrolase fold domain~~, ~~a helical cap domain~~, ~~and a jellyroll beta-domain. Structural homology was observed to~~ the ~~Rhodococcus cocaine esterase, indicating that both enzymes belong~~ to the ~~same class of bacterial hydrolases~~. ~~Docking of a beta-lactam antibiotic in the active site explains the substrate specificity~~, ~~specifically~~ the ~~necessity of an alpha~~-~~amino group on the substrate, and explains the low specificity toward the beta~~-~~lactam nucleus.~~

The line below this paragraph, {{ABSTRACT_PUBMED_12684501}}, adds the Publication Abstract to the page

(as it appears on PubMed at http://www.pubmed.gov), where 12684501 is the PubMed ID number.

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==About this Structure==

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[[Category: Jellyroll]]

[[Category: Selenomethionine]]

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Sat May~~ 3 01:34:37 2008''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Jul 3 00:46:53 2008''

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-[[Image:1mpx.gif|left|200px]]
+{{Seed}}
+[[Image:1mpx.png|left|200px]]
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 {{STRUCTURE_1mpx|  PDB=1mpx  |  SCENE=  }}
-'''ALPHA-AMINO ACID ESTER HYDROLASE LABELED WITH SELENOMETHIONINE'''
+===ALPHA-AMINO ACID ESTER HYDROLASE LABELED WITH SELENOMETHIONINE===
-==Overview==
+<!--
-alpha-Amino acid ester hydrolases (AEHs) catalyze the hydrolysis and synthesis of esters and amides with an alpha-amino group. As such, they can synthesize beta-lactam antibiotics from acyl compounds and beta-lactam nuclei obtained from the hydrolysis of natural antibiotics. This article describes the gene sequence and the 1.9-A resolution crystal structure of the AEH from Xanthomonas citri. The enzyme consists of an alpha/beta-hydrolase fold domain, a helical cap domain, and a jellyroll beta-domain. Structural homology was observed to the Rhodococcus cocaine esterase, indicating that both enzymes belong to the same class of bacterial hydrolases. Docking of a beta-lactam antibiotic in the active site explains the substrate specificity, specifically the necessity of an alpha-amino group on the substrate, and explains the low specificity toward the beta-lactam nucleus.
+The line below this paragraph, {{ABSTRACT_PUBMED_12684501}}, adds the Publication Abstract to the page
+(as it appears on PubMed at http://www.pubmed.gov), where 12684501 is the PubMed ID number.
+-->
+{{ABSTRACT_PUBMED_12684501}}
 ==About this Structure==
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 [[Category: Jellyroll]]
 [[Category: Selenomethionine]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 01:34:37 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Jul  3 00:46:53 2008''