8tzr: Difference between revisions

Latest revision as of 14:13, 18 October 2023

Structure of human Wnt3a bound to WLS and CALR

Structural highlights

8tzr is a 3 chain structure with sequence from Homo sapiens. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	Electron Microscopy, Resolution 3.5Å
Ligands:	BMA, GLC, MAN, NAG, PAM, POV
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Disease

WNT3A_HUMAN Idiopathic juvenile osteoporosis.

Function

WNT3A_HUMAN Ligand for members of the frizzled family of seven transmembrane receptors (Probable). Functions in the canonical Wnt signaling pathway that results in activation of transcription factors of the TCF/LEF family (PubMed:20093360, PubMed:21244856, PubMed:24841207, PubMed:26902720). Required for normal embryonic mesoderm development and formation of caudal somites. Required for normal morphogenesis of the developing neural tube (By similarity). Mediates self-renewal of the stem cells at the bottom on intestinal crypts (in vitro) (PubMed:26902720).[UniProtKB:P27467]^[1] ^[2] ^[3] ^[4]

References

↑ Bourhis E, Tam C, Franke Y, Bazan JF, Ernst J, Hwang J, Costa M, Cochran AG, Hannoush RN. Reconstitution of a frizzled8.Wnt3a.LRP6 signaling complex reveals multiple Wnt and Dkk1 binding sites on LRP6. J Biol Chem. 2010 Mar 19;285(12):9172-9. PMID:20093360 doi:10.1074/jbc.M109.092130
↑ Doubravska L, Krausova M, Gradl D, Vojtechova M, Tumova L, Lukas J, Valenta T, Pospichalova V, Fafilek B, Plachy J, Sebesta O, Korinek V. Fatty acid modification of Wnt1 and Wnt3a at serine is prerequisite for lipidation at cysteine and is essential for Wnt signalling. Cell Signal. 2011 May;23(5):837-48. PMID:21244856 doi:10.1016/j.cellsig.2011.01.007
↑ MacDonald BT, Hien A, Zhang X, Iranloye O, Virshup DM, Waterman ML, He X. Disulfide bond requirements for active Wnt ligands. J Biol Chem. 2014 Jun 27;289(26):18122-36. PMID:24841207 doi:10.1074/jbc.M114.575027
↑ Mihara E, Hirai H, Yamamoto H, Tamura-Kawakami K, Matano M, Kikuchi A, Sato T, Takagi J. Active and water-soluble form of lipidated Wnt protein is maintained by a serum glycoprotein afamin/alpha-albumin. Elife. 2016 Feb 23;5. doi: 10.7554/eLife.11621. PMID:26902720 doi:https://dx.doi.org/10.7554/eLife.11621

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OCA

[1] Bourhis E, Tam C, Franke Y, Bazan JF, Ernst J, Hwang J, Costa M, Cochran AG, Hannoush RN. Reconstitution of a frizzled8.Wnt3a.LRP6 signaling complex reveals multiple Wnt and Dkk1 binding sites on LRP6. J Biol Chem. 2010 Mar 19;285(12):9172-9. PMID:20093360 doi:10.1074/jbc.M109.092130

[2] Doubravska L, Krausova M, Gradl D, Vojtechova M, Tumova L, Lukas J, Valenta T, Pospichalova V, Fafilek B, Plachy J, Sebesta O, Korinek V. Fatty acid modification of Wnt1 and Wnt3a at serine is prerequisite for lipidation at cysteine and is essential for Wnt signalling. Cell Signal. 2011 May;23(5):837-48. PMID:21244856 doi:10.1016/j.cellsig.2011.01.007

[3] MacDonald BT, Hien A, Zhang X, Iranloye O, Virshup DM, Waterman ML, He X. Disulfide bond requirements for active Wnt ligands. J Biol Chem. 2014 Jun 27;289(26):18122-36. PMID:24841207 doi:10.1074/jbc.M114.575027

[4] Mihara E, Hirai H, Yamamoto H, Tamura-Kawakami K, Matano M, Kikuchi A, Sato T, Takagi J. Active and water-soluble form of lipidated Wnt protein is maintained by a serum glycoprotein afamin/alpha-albumin. Elife. 2016 Feb 23;5. doi: 10.7554/eLife.11621. PMID:26902720 doi:https://dx.doi.org/10.7554/eLife.11621

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@@ Line 1: / Line 1: @@
-'''Unreleased structure'''
-The entry 8tzr is ON HOLD  until Paper Publication
+==Structure of human Wnt3a bound to WLS and CALR==
+<StructureSection load='8tzr' size='340' side='right'caption='[[8tzr]], [[Resolution|resolution]] 3.50&Aring;' scene=''>
-Authors:
+== Structural highlights ==
+<table><tr><td colspan='2'>[[8tzr]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8TZR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8TZR FirstGlance]. <br>
-Description:
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.5&#8491;</td></tr>
-[[Category: Unreleased Structures]]
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=PAM:PALMITOLEIC+ACID'>PAM</scene>, <scene name='pdbligand=POV:(2S)-3-(HEXADECANOYLOXY)-2-[(9Z)-OCTADEC-9-ENOYLOXY]PROPYL+2-(TRIMETHYLAMMONIO)ETHYL+PHOSPHATE'>POV</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8tzr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8tzr OCA], [https://pdbe.org/8tzr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8tzr RCSB], [https://www.ebi.ac.uk/pdbsum/8tzr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8tzr ProSAT]</span></td></tr>
+</table>
+== Disease ==
+[https://www.uniprot.org/uniprot/WNT3A_HUMAN WNT3A_HUMAN] Idiopathic juvenile osteoporosis.
+== Function ==
+[https://www.uniprot.org/uniprot/WNT3A_HUMAN WNT3A_HUMAN] Ligand for members of the frizzled family of seven transmembrane receptors (Probable). Functions in the canonical Wnt signaling pathway that results in activation of transcription factors of the TCF/LEF family (PubMed:20093360, PubMed:21244856, PubMed:24841207, PubMed:26902720). Required for normal embryonic mesoderm development and formation of caudal somites. Required for normal morphogenesis of the developing neural tube (By similarity). Mediates self-renewal of the stem cells at the bottom on intestinal crypts (in vitro) (PubMed:26902720).[UniProtKB:P27467]<ref>PMID:20093360</ref> <ref>PMID:21244856</ref> <ref>PMID:24841207</ref> <ref>PMID:26902720</ref>
+== References ==
+<references/>
+__TOC__
+</StructureSection>
+[[Category: Homo sapiens]]
+[[Category: Large Structures]]
+[[Category: Hu Q]]
+[[Category: Li X]]
+[[Category: Qi X]]

8tzr: Difference between revisions

Latest revision as of 14:13, 18 October 2023

Structure of human Wnt3a bound to WLS and CALR

Structural highlights

Disease

Function

References

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