1naf: Difference between revisions

Line 1:

[[Image:1naf.gif|left|200px]]<br />

[[Image:1naf.gif|left|200px]]<br /><applet load="1naf" size="350" color="white" frame="true" align="right" spinBox="true"

<applet load="1naf" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

caption="1naf, resolution 2.80Å" />

'''crystal structure of the human GGA1 GAT domain'''<br />

==Overview==

The GGAs are a family of clathrin adaptor proteins involved in vesicular, transport between the trans-Golgi network and endosomal system. Here we, confirm reports that GGAs are targeted to the Golgi via interaction, between the GGA-GAT domain and ARF-GTP, and we present the structure of, the GAT domain of human GGA1, completing the structural description of the, folded domains of GGA proteins. The GGA-GAT domain possesses an all, alpha-helical fold with a "paper clip" topology comprising two independent, subdomains. Structure-based mutagenesis demonstrates that ARF1-GTP binding, by GGAs is exclusively governed by the N-terminal "hook" subdomain, and, using an in vitro recruitment assay, we show that ARF-GTP binding by this, small structure is required and sufficient for Golgi targeting of GGAs.

The GGAs are a family of clathrin adaptor proteins involved in vesicular transport between the trans-Golgi network and endosomal system. Here we confirm reports that GGAs are targeted to the Golgi via interaction between the GGA-GAT domain and ARF-GTP, and we present the structure of the GAT domain of human GGA1, completing the structural description of the folded domains of GGA proteins. The GGA-GAT domain possesses an all alpha-helical fold with a "paper clip" topology comprising two independent subdomains. Structure-based mutagenesis demonstrates that ARF1-GTP binding by GGAs is exclusively governed by the N-terminal "hook" subdomain, and, using an in vitro recruitment assay, we show that ARF-GTP binding by this small structure is required and sufficient for Golgi targeting of GGAs.

==About this Structure==

1NAF is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=1NAF OCA].

1NAF is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NAF OCA].

==Reference==

Line 14:

Line 13:

[[Category: Homo sapiens]]

[[Category: Single protein]]

[[Category: Collins, B.M.]]

[[Category: Collins, B M.]]

[[Category: Owen, D.J.]]

[[Category: Owen, D J.]]

[[Category: Watson, P.J.]]

[[Category: Watson, P J.]]

[[Category: clathrin-adaptor]]

[[Category: gat domain]]

Line 23:

Line 22:

[[Category: three-helix bundle]]

''Page seeded by [http://~~ispc~~.weizmann.ac.il/oca OCA ] on ~~Mon Nov 12 18~~:19:~~44 2007~~''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:03:58 2008''

Navigation menu

Page actions

Page actions

Personal tools

Navigation

Search

Tools

@@ Line 1: / Line 1: @@
-[[Image:1naf.gif|left|200px]]<br />
+[[Image:1naf.gif|left|200px]]<br /><applet load="1naf" size="350" color="white" frame="true" align="right" spinBox="true"
-<applet load="1naf" size="450" color="white" frame="true" align="right" spinBox="true"
 caption="1naf, resolution 2.80&Aring;" />
 '''crystal structure of the human GGA1 GAT domain'''<br />
 ==Overview==
-The GGAs are a family of clathrin adaptor proteins involved in vesicular, transport between the trans-Golgi network and endosomal system. Here we, confirm reports that GGAs are targeted to the Golgi via interaction, between the GGA-GAT domain and ARF-GTP, and we present the structure of, the GAT domain of human GGA1, completing the structural description of the, folded domains of GGA proteins. The GGA-GAT domain possesses an all, alpha-helical fold with a "paper clip" topology comprising two independent, subdomains. Structure-based mutagenesis demonstrates that ARF1-GTP binding, by GGAs is exclusively governed by the N-terminal "hook" subdomain, and, using an in vitro recruitment assay, we show that ARF-GTP binding by this, small structure is required and sufficient for Golgi targeting of GGAs.
+The GGAs are a family of clathrin adaptor proteins involved in vesicular transport between the trans-Golgi network and endosomal system. Here we confirm reports that GGAs are targeted to the Golgi via interaction between the GGA-GAT domain and ARF-GTP, and we present the structure of the GAT domain of human GGA1, completing the structural description of the folded domains of GGA proteins. The GGA-GAT domain possesses an all alpha-helical fold with a "paper clip" topology comprising two independent subdomains. Structure-based mutagenesis demonstrates that ARF1-GTP binding by GGAs is exclusively governed by the N-terminal "hook" subdomain, and, using an in vitro recruitment assay, we show that ARF-GTP binding by this small structure is required and sufficient for Golgi targeting of GGAs.
 ==About this Structure==
-NAF is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1NAF OCA].
+NAF is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NAF OCA].
 ==Reference==
@@ Line 14: / Line 13: @@
 [[Category: Homo sapiens]]
 [[Category: Single protein]]
-[[Category: Collins, B.M.]]
+[[Category: Collins, B M.]]
-[[Category: Owen, D.J.]]
+[[Category: Owen, D J.]]
-[[Category: Watson, P.J.]]
+[[Category: Watson, P J.]]
 [[Category: clathrin-adaptor]]
 [[Category: gat domain]]
@@ Line 23: / Line 22: @@
 [[Category: three-helix bundle]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 12 18:19:44 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:03:58 2008''