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| <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5jva FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5jva OCA], [https://pdbe.org/5jva PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5jva RCSB], [https://www.ebi.ac.uk/pdbsum/5jva PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5jva ProSAT]</span></td></tr> | | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5jva FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5jva OCA], [https://pdbe.org/5jva PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5jva RCSB], [https://www.ebi.ac.uk/pdbsum/5jva PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5jva ProSAT]</span></td></tr> |
| </table> | | </table> |
| <div style="background-color:#fffaf0;">
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| == Publication Abstract from PubMed ==
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| The red fluorescent protein variant TagRFP-T has greatly improved photostability over its parent molecule, TagRFP, but the underlying mechanism leading to this improvement is to date unknown. The 1.95 A resolution crystallographic structure of TagRFP-T showed that its chromophore exists as a mixture of cis and trans coplanar isomers in roughly equal proportions. Interestingly, both isomers are able to fluoresce, a property that has never been observed in any other fluorescent protein. We propose a "circular restoration model" for TagRFP-T to explain its superior photostability: There are four co-existing chromophore states (cis/trans protonated/ionized state) that can be driven by light to transform from one state into another. This model also explains how TagRPF-T essentially eliminates the temporary dark state (reversible photobleaching).
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| The crystal structure of red fluorescent protein TagRFP-T reveals the mechanism of its superior photostability.,Liu R, Liang QN, Du SQ, Hu XJ, Ding Y Biochem Biophys Res Commun. 2016 Aug 19;477(2):229-34. doi:, 10.1016/j.bbrc.2016.06.047. Epub 2016 Jun 11. PMID:27297107<ref>PMID:27297107</ref>
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| From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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| </div>
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| <div class="pdbe-citations 5jva" style="background-color:#fffaf0;"></div>
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| == References ==
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| <references/>
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| __TOC__ | | __TOC__ |
| </StructureSection> | | </StructureSection> |
