1n33: Difference between revisions

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[[Image:1n33.~~gif~~|left|200px]]

[[Image:1n33.png|left|200px]]

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~~'''~~Structure of the Thermus thermophilus 30S ribosomal subunit bound to codon and near-cognate transfer rna anticodon stem-loop mismatched at the second codon position at the a site with paromomycin~~'''~~

===Structure of the Thermus thermophilus 30S ribosomal subunit bound to codon and near-cognate transfer rna anticodon stem-loop mismatched at the second codon position at the a site with paromomycin===

~~==Overview==~~

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~~A structural and mechanistic explanation for~~ the ~~selection of tRNAs by~~ the ~~ribosome has been elusive. Here, we report crystal structures of the 30S ribosomal subunit with codon and near-cognate tRNA anticodon stem loops bound~~ at ~~the decoding center and compare affinities of equivalent complexes in solution~~. ~~In ribosomal interactions with near-cognate tRNA, deviation from Watson-Crick geometry results in uncompensated desolvation of hydrogen-bonding partners at the codon-anticodon minor groove~~. ~~As a result~~, ~~the transition to a closed form of the 30S induced by cognate tRNA~~ is ~~unfavorable for near-cognate tRNA unless paromomycin induces part of~~ the ~~rearrangement. We conclude that stabilization of a closed 30S conformation is required for tRNA selection, and thereby structurally rationalize much previous data on translational fidelity~~.

The line below this paragraph, {{ABSTRACT_PUBMED_12464183}}, adds the Publication Abstract to the page

(as it appears on PubMed at http://www.pubmed.gov), where 12464183 is the PubMed ID number.

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==About this Structure==

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[[Category: Trna]]

[[Category: Wobble]]

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Sat May 3 02~~:01:48 2008''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 23:48:08 2008''

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-[[Image:1n33.gif|left|200px]]
+{{Seed}}
+[[Image:1n33.png|left|200px]]
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 {{STRUCTURE_1n33|  PDB=1n33  |  SCENE=  }}
-'''Structure of the Thermus thermophilus 30S ribosomal subunit bound to codon and near-cognate transfer rna anticodon stem-loop mismatched at the second codon position at the a site with paromomycin'''
+===Structure of the Thermus thermophilus 30S ribosomal subunit bound to codon and near-cognate transfer rna anticodon stem-loop mismatched at the second codon position at the a site with paromomycin===
-==Overview==
+<!--
-A structural and mechanistic explanation for the selection of tRNAs by the ribosome has been elusive. Here, we report crystal structures of the 30S ribosomal subunit with codon and near-cognate tRNA anticodon stem loops bound at the decoding center and compare affinities of equivalent complexes in solution. In ribosomal interactions with near-cognate tRNA, deviation from Watson-Crick geometry results in uncompensated desolvation of hydrogen-bonding partners at the codon-anticodon minor groove. As a result, the transition to a closed form of the 30S induced by cognate tRNA is unfavorable for near-cognate tRNA unless paromomycin induces part of the rearrangement. We conclude that stabilization of a closed 30S conformation is required for tRNA selection, and thereby structurally rationalize much previous data on translational fidelity.
+The line below this paragraph, {{ABSTRACT_PUBMED_12464183}}, adds the Publication Abstract to the page
+(as it appears on PubMed at http://www.pubmed.gov), where 12464183 is the PubMed ID number.
+-->
+{{ABSTRACT_PUBMED_12464183}}
 ==About this Structure==
@@ Line 44: / Line 48: @@
 [[Category: Trna]]
 [[Category: Wobble]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 02:01:48 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 23:48:08 2008''