1na5: Difference between revisions

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[[Image:1na5.gif|left|200px]]
{{Seed}}
[[Image:1na5.png|left|200px]]


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{{STRUCTURE_1na5|  PDB=1na5  |  SCENE=  }}  
{{STRUCTURE_1na5|  PDB=1na5  |  SCENE=  }}  


'''INTEGRIN ALPHA M I DOMAIN'''
===INTEGRIN ALPHA M I DOMAIN===




==Overview==
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The alpha-I domain, found in the alpha-subunit of the leucocyte integrins such as alphaMbeta2 and alphaLbeta2, switches between the open and closed tertiary conformations, reflecting the high- and low-affinity ligand-binding states of the integrin that are required for regulated cell adhesion and migration. In the present study we show, by using point mutations and engineered disulphide bonds, that ligand affinity can be reduced or increased allosterically by altering the equilibrium between the closed and open states. We determined equilibrium constants for the binding of two ligands, fibrinogen and intercellular cell-adhesion molecule 1, to the alphaM-I domain by surface plasmon resonance, and determined crystal structures of a low-affinity mutant. Locking the domain in the open conformation increases affinity by a factor of no greater than 10, consistent with a closely balanced equilibrium between the two conformations in the absence of ligand. This behaviour contrasts with that of the unliganded alphaL-I domain, for which the equilibrium lies strongly in favour of the closed conformation. These results suggest significant differences in the way the parent integrins regulate I domain conformation and hence ligand affinity.
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{{ABSTRACT_PUBMED_12611591}}


==About this Structure==
==About this Structure==
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[[Category: McCleverty, C J.]]
[[Category: McCleverty, C J.]]
[[Category: Rossmann fold]]
[[Category: Rossmann fold]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 14:08:14 2008''

Revision as of 11:08, 29 July 2008

File:1na5.png

Template:STRUCTURE 1na5

INTEGRIN ALPHA M I DOMAIN

Template:ABSTRACT PUBMED 12611591

About this Structure

1NA5 is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Engineered allosteric mutants of the integrin alphaMbeta2 I domain: structural and functional studies., McCleverty CJ, Liddington RC, Biochem J. 2003 May 15;372(Pt 1):121-7. PMID:12611591

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