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| [[Image:1nar.gif|left|200px]] | | {{Seed}} |
| | [[Image:1nar.png|left|200px]] |
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| {{STRUCTURE_1nar| PDB=1nar | SCENE= }} | | {{STRUCTURE_1nar| PDB=1nar | SCENE= }} |
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| '''CRYSTAL STRUCTURE OF NARBONIN REFINED AT 1.8 ANGSTROMS RESOLUTION'''
| | ===CRYSTAL STRUCTURE OF NARBONIN REFINED AT 1.8 ANGSTROMS RESOLUTION=== |
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| ==Overview==
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| The three-dimensional structure of narbonin, a seed protein from Vicia narbonensis L, has been determined at 1.8 A resolution. Phase information was obtained by multiple isomorphous replacement and optimized anomalous dispersion. The narbonin structure was initially traced with only 17% amino-acid sequence information and preliminarily refined to a crystallographic R-factor of 16.5%. It is now refined to 15.9% using full sequence information derived from cDNA and after the addition of more solvent molecules. The monomeric molecule of narbonin is an eight-stranded parallel beta-barrel surrounded by alpha-helices in a beta/alpha-topology similar to that first observed in triose phosphate isomerase. Differences exist in the N-terminal part of the polypeptide chain, where the first helix is replaced by a loop and the second beta-strand is followed by an additional antiparallel alpha-sheet placed parallel on top of alpha-helices alpha3 and alpha4. Two short additional secondary structures are present. The first, an alpha-helix, is situated between the seventh beta-strand and the following helix, and the second, which is a 3(10) helix, between the eighth strand and the C-terminal helix. The most striking observation is the lack of a known enzymatic function for narbonin, because all TIM-like structures known so far are enzymes. | | The line below this paragraph, {{ABSTRACT_PUBMED_15299319}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 15299319 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_15299319}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: Wilson, K S.]] | | [[Category: Wilson, K S.]] |
| [[Category: Plant seed protein]] | | [[Category: Plant seed protein]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 02:18:14 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 20:18:53 2008'' |
