1oct: Difference between revisions

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[[Image:1oct.gif|left|200px]]<br />

[[Image:1oct.gif|left|200px]]<br /><applet load="1oct" size="350" color="white" frame="true" align="right" spinBox="true"

<applet load="1oct" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

caption="1oct, resolution 3.000Å" />

'''CRYSTAL STRUCTURE OF THE OCT-1 POU DOMAIN BOUND TO AN OCTAMER SITE: DNA RECOGNITION WITH TETHERED DNA-BINDING MODULES'''<br />

==Overview==

The structure of an Oct-1 POU domain-octamer DNA complex has been solved, at 3.0 A resolution. The POU-specific domain contacts the 5' half of this, site (ATGCAAAT), and as predicted from nuclear magnetic resonance studies, the structure, docking, and contacts are remarkably similar to those of, the lambda and 434 repressors. The POU homeodomain contacts the 3' half of, this site (ATGCAAAT), and the docking is similar to that of the engrailed, MAT alpha 2, and Antennapedia homeodomains. The linker region is not, visible and there are no protein-protein contacts between the domains, but, overlapping phosphate contacts near the center of the octamer site may, favor cooperative binding. This novel arrangement raises important, questions about cooperativity in protein-DNA recognition.

The structure of an Oct-1 POU domain-octamer DNA complex has been solved at 3.0 A resolution. The POU-specific domain contacts the 5' half of this site (ATGCAAAT), and as predicted from nuclear magnetic resonance studies, the structure, docking, and contacts are remarkably similar to those of the lambda and 434 repressors. The POU homeodomain contacts the 3' half of this site (ATGCAAAT), and the docking is similar to that of the engrailed, MAT alpha 2, and Antennapedia homeodomains. The linker region is not visible and there are no protein-protein contacts between the domains, but overlapping phosphate contacts near the center of the octamer site may favor cooperative binding. This novel arrangement raises important questions about cooperativity in protein-DNA recognition.

==About this Structure==

1OCT is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=1OCT OCA].

1OCT is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OCT OCA].

==Reference==

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[[Category: Aurora, R.]]

[[Category: Herr, W.]]

[[Category: Klemm, J.D.]]

[[Category: Klemm, J D.]]

[[Category: Pabo, C.O.]]

[[Category: Pabo, C O.]]

[[Category: Rould, M.A.]]

[[Category: Rould, M A.]]

[[Category: protein-dna complex]]

''Page seeded by [http://~~ispc~~.weizmann.ac.il/oca OCA ] on ~~Mon Nov 12 18~~:31:~~31 2007~~''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:16:10 2008''

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@@ Line 1: / Line 1: @@
-[[Image:1oct.gif|left|200px]]<br />
+[[Image:1oct.gif|left|200px]]<br /><applet load="1oct" size="350" color="white" frame="true" align="right" spinBox="true"
-<applet load="1oct" size="450" color="white" frame="true" align="right" spinBox="true"
 caption="1oct, resolution 3.000&Aring;" />
 '''CRYSTAL STRUCTURE OF THE OCT-1 POU DOMAIN BOUND TO AN OCTAMER SITE: DNA RECOGNITION WITH TETHERED DNA-BINDING MODULES'''<br />
 ==Overview==
-The structure of an Oct-1 POU domain-octamer DNA complex has been solved, at 3.0 A resolution. The POU-specific domain contacts the 5' half of this, site (ATGCAAAT), and as predicted from nuclear magnetic resonance studies, the structure, docking, and contacts are remarkably similar to those of, the lambda and 434 repressors. The POU homeodomain contacts the 3' half of, this site (ATGCAAAT), and the docking is similar to that of the engrailed, MAT alpha 2, and Antennapedia homeodomains. The linker region is not, visible and there are no protein-protein contacts between the domains, but, overlapping phosphate contacts near the center of the octamer site may, favor cooperative binding. This novel arrangement raises important, questions about cooperativity in protein-DNA recognition.
+The structure of an Oct-1 POU domain-octamer DNA complex has been solved at 3.0 A resolution. The POU-specific domain contacts the 5' half of this site (ATGCAAAT), and as predicted from nuclear magnetic resonance studies, the structure, docking, and contacts are remarkably similar to those of the lambda and 434 repressors. The POU homeodomain contacts the 3' half of this site (ATGCAAAT), and the docking is similar to that of the engrailed, MAT alpha 2, and Antennapedia homeodomains. The linker region is not visible and there are no protein-protein contacts between the domains, but overlapping phosphate contacts near the center of the octamer site may favor cooperative binding. This novel arrangement raises important questions about cooperativity in protein-DNA recognition.
 ==About this Structure==
-OCT is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1OCT OCA].
+OCT is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OCT OCA].
 ==Reference==
@@ Line 16: / Line 15: @@
 [[Category: Aurora, R.]]
 [[Category: Herr, W.]]
-[[Category: Klemm, J.D.]]
+[[Category: Klemm, J D.]]
-[[Category: Pabo, C.O.]]
+[[Category: Pabo, C O.]]
-[[Category: Rould, M.A.]]
+[[Category: Rould, M A.]]
 [[Category: protein-dna complex]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 12 18:31:31 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:16:10 2008''