1np0: Difference between revisions

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[[Image:1np0.gif|left|200px]]
{{Seed}}
[[Image:1np0.png|left|200px]]


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{{STRUCTURE_1np0|  PDB=1np0  |  SCENE=  }}  
{{STRUCTURE_1np0|  PDB=1np0  |  SCENE=  }}  


'''Human lysosomal beta-hexosaminidase isoform B in complex with intermediate analogue NAG-thiazoline'''
===Human lysosomal beta-hexosaminidase isoform B in complex with intermediate analogue NAG-thiazoline===




==Overview==
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In humans, two major beta-hexosaminidase isoenzymes exist: Hex A and Hex B. Hex A is a heterodimer of subunits alpha and beta (60% identity), whereas Hex B is a homodimer of beta-subunits. Interest in human beta-hexosaminidase stems from its association with Tay-Sachs and Sandhoff disease; these are prototypical lysosomal storage disorders resulting from the abnormal accumulation of G(M2)-ganglioside (G(M2)). Hex A degrades G(M2) by removing a terminal N-acetyl-D-galactosamine (beta-GalNAc) residue, and this activity requires the G(M2)-activator, a protein which solubilizes the ganglioside for presentation to Hex A. We present here the crystal structure of human Hex B, alone (2.4A) and in complex with the mechanistic inhibitors GalNAc-isofagomine (2.2A) or NAG-thiazoline (2.5A). From these, and the known X-ray structure of the G(M2)-activator, we have modeled Hex A in complex with the activator and ganglioside. Together, our crystallographic and modeling data demonstrate how alpha and beta-subunits dimerize to form either Hex A or Hex B, how these isoenzymes hydrolyze diverse substrates, and how many documented point mutations cause Sandhoff disease (beta-subunit mutations) and Tay-Sachs disease (alpha-subunit mutations).
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{{ABSTRACT_PUBMED_12662933}}


==About this Structure==
==About this Structure==
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[[Category: Family 20 glycosidase]]
[[Category: Family 20 glycosidase]]
[[Category: Homodimer]]
[[Category: Homodimer]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 02:48:00 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 23:10:47 2008''

Revision as of 20:10, 28 July 2008

File:1np0.png

Template:STRUCTURE 1np0

Human lysosomal beta-hexosaminidase isoform B in complex with intermediate analogue NAG-thiazoline

Template:ABSTRACT PUBMED 12662933

About this Structure

1NP0 is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Crystal structure of human beta-hexosaminidase B: understanding the molecular basis of Sandhoff and Tay-Sachs disease., Mark BL, Mahuran DJ, Cherney MM, Zhao D, Knapp S, James MN, J Mol Biol. 2003 Apr 11;327(5):1093-109. PMID:12662933

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