1nt9: Difference between revisions

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[[Image:1nt9.gif|left|200px]]
{{Seed}}
[[Image:1nt9.png|left|200px]]


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{{STRUCTURE_1nt9|  PDB=1nt9  |  SCENE=  }}  
{{STRUCTURE_1nt9|  PDB=1nt9  |  SCENE=  }}  


'''Complete 12-subunit RNA polymerase II'''
===Complete 12-subunit RNA polymerase II===




==Overview==
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RNA polymerase (Pol) II consists of a 10-polypeptide catalytic core and the two-subunit Rpb4/7 complex that is required for transcription initiation. Previous structures of the Pol II core revealed a "clamp," which binds the DNA template strand via three "switch regions," and a flexible "linker" to the C-terminal repeat domain (CTD). Here we derived a model of the complete Pol II by fitting structures of the core and Rpb4/7 to a 4.2-A crystallographic electron density map. Rpb4/7 protrudes from the polymerase "upstream face," on which initiation factors assemble for promoter DNA loading. Rpb7 forms a wedge between the clamp and the linker, restricting the clamp to a closed position. The wedge allosterically prevents entry of the promoter DNA duplex into the active center cleft and induces in two switch regions a conformation poised for template-strand binding. Interaction of Rpb4/7 with the linker explains Rpb4-mediated recruitment of the CTD phosphatase to the CTD during Pol II recycling. The core-Rpb7 interaction and some functions of Rpb4/7 are apparently conserved in all eukaryotic and archaeal RNA polymerases but not in the bacterial enzyme.
The line below this paragraph, {{ABSTRACT_PUBMED_12746495}}, adds the Publication Abstract to the page
(as it appears on PubMed at http://www.pubmed.gov), where 12746495 is the PubMed ID number.
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{{ABSTRACT_PUBMED_12746495}}


==About this Structure==
==About this Structure==
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[[Category: Multisubunit complex]]
[[Category: Multisubunit complex]]
[[Category: Transcription]]
[[Category: Transcription]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 02:57:03 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 12:40:48 2008''

Revision as of 09:40, 27 July 2008

File:1nt9.png

Template:STRUCTURE 1nt9

Complete 12-subunit RNA polymerase II

Template:ABSTRACT PUBMED 12746495

About this Structure

1NT9 is a Protein complex structure of sequences from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.

Reference

Architecture of initiation-competent 12-subunit RNA polymerase II., Armache KJ, Kettenberger H, Cramer P, Proc Natl Acad Sci U S A. 2003 Jun 10;100(12):6964-8. Epub 2003 May 13. PMID:12746495

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