1nwh: Difference between revisions

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[[Image:1nwh.gif|left|200px]]
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[[Image:1nwh.png|left|200px]]


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{{STRUCTURE_1nwh|  PDB=1nwh  |  SCENE=  }}  
{{STRUCTURE_1nwh|  PDB=1nwh  |  SCENE=  }}  


'''Crystal Structure of Aspartate Semialdehyde Dehydrogenase from Haemophilus influenzae as a Tetrahedral Hemithioacetal Reaction Intermediate at 2.0 A'''
===Crystal Structure of Aspartate Semialdehyde Dehydrogenase from Haemophilus influenzae as a Tetrahedral Hemithioacetal Reaction Intermediate at 2.0 A===




==Overview==
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The structural analysis of an enzymatic reaction intermediate affords a unique opportunity to study a catalytic mechanism in extraordinary detail. Here we present the structure of a tetrahedral intermediate in the catalytic cycle of aspartate-beta-semialdehyde dehydrogenase (ASADH) from Haemophilus influenzae at 2.0-A resolution. ASADH is not found in humans, yet its catalytic activity is required for the biosynthesis of essential amino acids in plants and microorganisms. Diaminopimelic acid, also formed by this enzymatic pathway, is an integral component of bacterial cell walls, thus making ASADH an attractive target for the development of new antibiotics. This enzyme is able to capture the substrates aspartate-beta-semialdehyde and phosphate as an active complex that does not complete the catalytic cycle in the absence of NADP. A distinctive binding pocket in which the hemithioacetal oxygen of the bound substrate is stabilized by interaction with a backbone amide group dictates the R stereochemistry of the tetrahedral intermediate. This pocket, reminiscent of the oxyanion hole found in serine proteases, is completed through hydrogen bonding to the bound phosphate substrate.
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==About this Structure==
==About this Structure==
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[[Category: Hemithioacetal]]
[[Category: Hemithioacetal]]
[[Category: Tetrahedral intermediate]]
[[Category: Tetrahedral intermediate]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 21:19:16 2008''

Revision as of 18:19, 28 July 2008

File:1nwh.png

Template:STRUCTURE 1nwh

Crystal Structure of Aspartate Semialdehyde Dehydrogenase from Haemophilus influenzae as a Tetrahedral Hemithioacetal Reaction Intermediate at 2.0 A

Template:ABSTRACT PUBMED 14559965

About this Structure

1NWH is a Single protein structure of sequence from Haemophilus influenzae. Full crystallographic information is available from OCA.

Reference

Capture of an intermediate in the catalytic cycle of L-aspartate-beta-semialdehyde dehydrogenase., Blanco J, Moore RA, Viola RE, Proc Natl Acad Sci U S A. 2003 Oct 28;100(22):12613-7. Epub 2003 Oct 14. PMID:14559965

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