1ou5: Difference between revisions
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'''Crystal structure of human CCA-adding enzyme'''<br /> | '''Crystal structure of human CCA-adding enzyme'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
1OU5 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http:// | 1OU5 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OU5 OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: trna]] | [[Category: trna]] | ||
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Revision as of 14:35, 15 February 2008
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Crystal structure of human CCA-adding enzyme
Overview
All tRNA molecules carry the invariant sequence CCA at their 3'-terminus, for amino acid attachment. The post-transcriptional addition of CCA is, carried out by ATP(CTP):tRNA nucleotidyltransferase, also called CCase., This enzyme catalyses a unique template-independent but sequence-specific, nucleotide polymerization reaction. In order to reveal the molecular, mechanism of this activity, we solved the crystal structure of human CCase, by single isomorphous replacement. The structure reveals a four domain, architecture with a cluster of conserved residues forming a positively, charged cleft between the first two domains. Structural homology of the, N-terminal CCase domain to other nucleotidyltransferases could be, exploited for modeling a tRNA-substrate complex. The model places the tRNA, 3'-end into the N-terminal nucleotidyltransferase site, close to a patch, of conserved residues that provide the binding sites for CTP and ATP., Based on our results, we introduce a corkscrew model for CCA addition that, includes a fixed active site and a traveling tRNA-binding region formed by, flexible parts of the protein.
Disease
Known disease associated with this structure: Deafness, mitochondrial, modifier of OMIM:[610230]
About this Structure
1OU5 is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Crystal structure of the human CCA-adding enzyme: insights into template-independent polymerization., Augustin MA, Reichert AS, Betat H, Huber R, Morl M, Steegborn C, J Mol Biol. 2003 May 16;328(5):985-94. PMID:12729736
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