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| <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2kyd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2kyd OCA], [https://pdbe.org/2kyd PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2kyd RCSB], [https://www.ebi.ac.uk/pdbsum/2kyd PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2kyd ProSAT]</span></td></tr> | | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2kyd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2kyd OCA], [https://pdbe.org/2kyd PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2kyd RCSB], [https://www.ebi.ac.uk/pdbsum/2kyd PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2kyd ProSAT]</span></td></tr> |
| </table> | | </table> |
| <div style="background-color:#fffaf0;">
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| == Publication Abstract from PubMed ==
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| Ribonucleic acid structure determination by NMR spectroscopy relies primarily on local structural restraints provided by (1)H- (1)H NOEs and J-couplings. When employed loosely, these restraints are broadly compatible with A- and B-like helical geometries and give rise to calculated structures that are highly sensitive to the force fields employed during refinement. A survey of recently reported NMR structures reveals significant variations in helical parameters, particularly the major groove width. Although helical parameters observed in high-resolution X-ray crystal structures of isolated A-form RNA helices are sensitive to crystal packing effects, variations among the published X-ray structures are significantly smaller than those observed in NMR structures. Here we show that restraints derived from aromatic (1)H- (13)C residual dipolar couplings (RDCs) and residual chemical shift anisotropies (RCSAs) can overcome NMR restraint and force field deficiencies and afford structures with helical properties similar to those observed in high-resolution X-ray structures.
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| Major groove width variations in RNA structures determined by NMR and impact of 13C residual chemical shift anisotropy and 1H-13C residual dipolar coupling on refinement.,Tolbert BS, Miyazaki Y, Barton S, Kinde B, Starck P, Singh R, Bax A, Case DA, Summers MF J Biomol NMR. 2010 Jul;47(3):205-19. Epub 2010 Jun 15. PMID:20549304<ref>PMID:20549304</ref>
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| From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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| </div>
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| <div class="pdbe-citations 2kyd" style="background-color:#fffaf0;"></div>
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| == References ==
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| <references/>
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| __TOC__ | | __TOC__ |
| </StructureSection> | | </StructureSection> |
