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| <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2l3e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2l3e OCA], [https://pdbe.org/2l3e PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2l3e RCSB], [https://www.ebi.ac.uk/pdbsum/2l3e PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2l3e ProSAT]</span></td></tr> | | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2l3e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2l3e OCA], [https://pdbe.org/2l3e PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2l3e RCSB], [https://www.ebi.ac.uk/pdbsum/2l3e PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2l3e ProSAT]</span></td></tr> |
| </table> | | </table> |
| <div style="background-color:#fffaf0;">
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| == Publication Abstract from PubMed ==
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| Telomerase is a unique ribonucleoprotein complex that catalyzes the addition of telomeric DNA repeats onto the 3' ends of linear chromosomes. All vertebrate telomerase RNAs contain a catalytically essential core domain that includes the template and a pseudoknot with extended helical subdomains. Within these helical regions is an asymmetric 5-nt internal bulge loop (J2a/b) flanked by helices (P2a and P2b) that is highly conserved in its location but not sequence. NMR structure determination reveals that J2a/b forms a defined S-shape and creates an approximately 90 degrees bend with a surprisingly low twist ( approximately 10 degrees ) between the flanking helices. A search of RNA structures revealed only one other example of a 5-nt bulge, from hepatitis C virus internal ribosome entry site, with a different sequence but the same structure. J2a/b is intrinsically flexible but the interhelical motions across the loop are remarkably restricted. Nucleotide substitutions in J2a/b that affect the bend angle, direction, and interhelical dynamics are correlated with telomerase activity. Based on the structures of P2ab (J2a/b and flanking helices), the conserved region of the pseudoknot (P2b/P3, previously determined) and the remaining helical segment (P2a.1-J2a.1 refined using residual dipolar couplings and the modeling program MC-Sym) we have calculated an NMR-based model of the full-length pseudoknot. The model and dynamics analysis show that J2a/b serves as a dominant structural and dynamical element in defining the overall topology of the core domain, and suggest that interhelical motions in P2ab facilitate nucleotide addition along the template and template translocation.
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| Structurally conserved five nucleotide bulge determines the overall topology of the core domain of human telomerase RNA.,Zhang Q, Kim NK, Peterson RD, Wang Z, Feigon J Proc Natl Acad Sci U S A. 2010 Nov 2;107(44):18761-8. Epub 2010 Oct 21. PMID:20966348<ref>PMID:20966348</ref>
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| From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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| </div>
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| <div class="pdbe-citations 2l3e" style="background-color:#fffaf0;"></div>
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| ==See Also== | | ==See Also== |
| *[[Telomerase 3D structures|Telomerase 3D structures]] | | *[[Telomerase 3D structures|Telomerase 3D structures]] |
| == References ==
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| <references/>
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| __TOC__ | | __TOC__ |
| </StructureSection> | | </StructureSection> |
