1oci: Difference between revisions

From Proteopedia
Jump to navigationJump to search
← Older editNewer edit →
OCA (talk | contribs)
Bots, Bureaucrats, journal, Administrators
3,330,058 edits
No edit summary
OCA (talk | contribs)
Bots, Bureaucrats, journal, Administrators
3,330,058 edits
No edit summary
Line 1: Line 1:
[[Image:1oci.gif|left|200px]]
{{Seed}}
[[Image:1oci.png|left|200px]]


<!--
<!--
Line 9: Line 10:
{{STRUCTURE_1oci|  PDB=1oci  |  SCENE=  }}  
{{STRUCTURE_1oci|  PDB=1oci  |  SCENE=  }}  


'''[3.2.0]BCANA:DNA'''
===[3.2.0]BCANA:DNA===




==Overview==
<!--  
[3.2.0]bcANA is a D-arabino-configured bicyclic nucleotide with a 2'-O,3'-C-methylene bridge. We here present the high-resolution NMR structure of a [3.2.0]bcANA modified dsDNA nonamer with one modified nucleotide incorporated. NOE restraints were obtained by analysis of NOESY cross peak intensities using a full relaxation matrix approach, and subsequently these restraints were incorporated into a simulated annealing scheme for the structure determination. In addition, the furanose ring puckers of the deoxyribose moieties were determined by analysis of COSY cross peaks. The modified duplex adopts a B-like geometry with Watson-Crick base pairing in all base pairs and all glycosidic angles in the anti range. The stacking arrangement of the nucleobases appears to be unperturbed relative to the normal B-like arrangement. The 2'-O,3'-C-methylene bridge of the modified nucleotide is located at the brim of the major groove where it fits well into the B-type duplex framework. The sugar pucker of the [3.2.0]bcANA nucleotide is O4'-endo and this sugar conformation causes a change in the delta backbone angle relative to the C2'-endo deoxyribose sugar pucker. This change is absorbed locally by slight changes in the epsilon and zeta angles of the modified nucleotide. Overall, the [3.2.0]bcANA modifications fits very well into a B-like duplex framework and only small and local perturbations are observed relative to the unmodified dsDNA of identical base sequence.
The line below this paragraph, {{ABSTRACT_PUBMED_12926371}}, adds the Publication Abstract to the page
(as it appears on PubMed at http://www.pubmed.gov), where 12926371 is the PubMed ID number.
-->
{{ABSTRACT_PUBMED_12926371}}


==About this Structure==
==About this Structure==
1OCI is a [[Single protein]] structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OCI OCA].  
1OCI is a [[Single protein]] structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OCI OCA].  


==Reference==
==Reference==
Line 32: Line 36:
[[Category: Nmr]]
[[Category: Nmr]]
[[Category: Rnase h]]
[[Category: Rnase h]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 03:39:58 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 12:15:38 2008''

Revision as of 09:15, 27 July 2008

File:1oci.png

Template:STRUCTURE 1oci

[3.2.0]BCANA:DNA

Template:ABSTRACT PUBMED 12926371

About this Structure

1OCI is a Single protein structure. Full experimental information is available from OCA.

Reference

NMR solution structure of dsDNA containing a bicyclic D-arabino-configured nucleotide fixed in an O4'-endo sugar conformation., Tommerholt HV, Christensen NK, Nielsen P, Wengel J, Stein PC, Jacobsen JP, Petersen M, Org Biomol Chem. 2003 May 21;1(10):1790-7. PMID:12926371

Page seeded by OCA on Sun Jul 27 12:15:38 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "https://proteopedia.org/index.php?title=1oci&oldid=645552"