1ocj: Difference between revisions

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[[Image:1ocj.~~jpg~~|left|200px]]

[[Image:1ocj.png|left|200px]]

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~~'''~~MUTANT D416A OF THE CELLOBIOHYDROLASE CEL6A FROM HUMICOLA INSOLENS IN COMPLEX WITH A THIOPENTASACCHARIDE AT 1.3 ANGSTROM RESOLUTION~~'''~~

===MUTANT D416A OF THE CELLOBIOHYDROLASE CEL6A FROM HUMICOLA INSOLENS IN COMPLEX WITH A THIOPENTASACCHARIDE AT 1.3 ANGSTROM RESOLUTION===

~~==Overview==~~

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The ~~enzymatic digestion of cellulose entails intimate involvement of cellobiohydrolases~~, ~~whose characteristic active-center tunnel contributes~~ to ~~a processive degradation of~~ the polysaccharide. The cellobiohydrolase Cel6A displays an active site within a tunnel formed by two extended loops, which are known to open and close in response to ligand binding. Here we present five structures of wild-type and mutant forms of Cel6A from Humicola insolens in complex with nonhydrolyzable thio-oligosaccharides, at ~~resolutions from 1~~.~~7-1~~.~~1 A~~, ~~dissecting the structural accommodation of a processing substrate chain through~~ the ~~active center during hydrolysis~~. ~~Movement of ligand is facilitated by extensive solvent~~-~~mediated interactions and through flexibility in the hydrophobic surfaces provided by a sheath of tryptophan residues.~~

The line below this paragraph, {{ABSTRACT_PUBMED_12842048}}, adds the Publication Abstract to the page

(as it appears on PubMed at http://www.pubmed.gov), where 12842048 is the PubMed ID number.

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==About this Structure==

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[[Category: Hydrolase]]

[[Category: Processive mechanism]]

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Sat May 3 03~~:40:03 2008''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 23:01:19 2008''

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-[[Image:1ocj.jpg|left|200px]]
+{{Seed}}
+[[Image:1ocj.png|left|200px]]
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 {{STRUCTURE_1ocj|  PDB=1ocj  |  SCENE=  }}
-'''MUTANT D416A OF THE CELLOBIOHYDROLASE CEL6A FROM HUMICOLA INSOLENS IN COMPLEX WITH A THIOPENTASACCHARIDE AT 1.3 ANGSTROM RESOLUTION'''
+===MUTANT D416A OF THE CELLOBIOHYDROLASE CEL6A FROM HUMICOLA INSOLENS IN COMPLEX WITH A THIOPENTASACCHARIDE AT 1.3 ANGSTROM RESOLUTION===
-==Overview==
+<!--
-The enzymatic digestion of cellulose entails intimate involvement of cellobiohydrolases, whose characteristic active-center tunnel contributes to a processive degradation of the polysaccharide. The cellobiohydrolase Cel6A displays an active site within a tunnel formed by two extended loops, which are known to open and close in response to ligand binding. Here we present five structures of wild-type and mutant forms of Cel6A from Humicola insolens in complex with nonhydrolyzable thio-oligosaccharides, at resolutions from 1.7-1.1 A, dissecting the structural accommodation of a processing substrate chain through the active center during hydrolysis. Movement of ligand is facilitated by extensive solvent-mediated interactions and through flexibility in the hydrophobic surfaces provided by a sheath of tryptophan residues.
+The line below this paragraph, {{ABSTRACT_PUBMED_12842048}}, adds the Publication Abstract to the page
+(as it appears on PubMed at http://www.pubmed.gov), where 12842048 is the PubMed ID number.
+-->
+{{ABSTRACT_PUBMED_12842048}}
 ==About this Structure==
@@ Line 36: / Line 40: @@
 [[Category: Hydrolase]]
 [[Category: Processive mechanism]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 03:40:03 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 23:01:19 2008''