1odh: Difference between revisions

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[[Image:1odh.~~gif~~|left|200px]]

[[Image:1odh.png|left|200px]]

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~~'''~~STRUCTURE OF THE GCM DOMAIN BOUND TO DNA~~'''~~

===STRUCTURE OF THE GCM DOMAIN BOUND TO DNA===

~~==Overview==~~

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~~Glia cell missing (GCM) transcription factors form a small family of transcriptional regulators in metazoans.~~ The ~~prototypical Drosophila GCM protein directs the differentiation of neuron precursor cells into glia cells~~, ~~whereas mammalian GCM proteins are involved in placenta and parathyroid development. GCM proteins share a highly conserved 150 amino acid residue region responsible for DNA binding~~, ~~known as~~ the ~~GCM domain. Here we present~~ the ~~crystal structure of the GCM domain from murine GCMa bound to its octameric DNA target site~~ at 2.~~85 A resolution~~. ~~The GCM domain exhibits a novel fold consisting of two domains tethered together by one of two structural Zn ions. We observe the novel use of a beta-sheet in DNA recognition~~, ~~whereby a five- stranded beta-sheet protrudes into the major groove perpendicular to~~ the ~~DNA axis~~. ~~The structure combined with mutational analysis of the target site and of DNA~~-~~contacting residues provides insight into DNA recognition by this new type of Zn~~-~~containing DNA-binding domain.~~

The line below this paragraph, {{ABSTRACT_PUBMED_12682016}}, adds the Publication Abstract to the page

(as it appears on PubMed at http://www.pubmed.gov), where 12682016 is the PubMed ID number.

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==About this Structure==

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[[Category: Protein/dna complex]]

[[Category: Transcription factor]]

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Sat May 3 03~~:42:23 2008''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 01:23:51 2008''

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-[[Image:1odh.gif|left|200px]]
+{{Seed}}
+[[Image:1odh.png|left|200px]]
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 {{STRUCTURE_1odh|  PDB=1odh  |  SCENE=  }}
-'''STRUCTURE OF THE GCM DOMAIN BOUND TO DNA'''
+===STRUCTURE OF THE GCM DOMAIN BOUND TO DNA===
-==Overview==
+<!--
-Glia cell missing (GCM) transcription factors form a small family of transcriptional regulators in metazoans. The prototypical Drosophila GCM protein directs the differentiation of neuron precursor cells into glia cells, whereas mammalian GCM proteins are involved in placenta and parathyroid development. GCM proteins share a highly conserved 150 amino acid residue region responsible for DNA binding, known as the GCM domain. Here we present the crystal structure of the GCM domain from murine GCMa bound to its octameric DNA target site at 2.85 A resolution. The GCM domain exhibits a novel fold consisting of two domains tethered together by one of two structural Zn ions. We observe the novel use of a beta-sheet in DNA recognition, whereby a five- stranded beta-sheet protrudes into the major groove perpendicular to the DNA axis. The structure combined with mutational analysis of the target site and of DNA-contacting residues provides insight into DNA recognition by this new type of Zn-containing DNA-binding domain.
+The line below this paragraph, {{ABSTRACT_PUBMED_12682016}}, adds the Publication Abstract to the page
+(as it appears on PubMed at http://www.pubmed.gov), where 12682016 is the PubMed ID number.
+-->
+{{ABSTRACT_PUBMED_12682016}}
 ==About this Structure==
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 [[Category: Protein/dna complex]]
 [[Category: Transcription factor]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 03:42:23 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 01:23:51 2008''