|
|
| Line 1: |
Line 1: |
| [[Image:1oij.jpg|left|200px]] | | {{Seed}} |
| | [[Image:1oij.png|left|200px]] |
|
| |
|
| <!-- | | <!-- |
| Line 9: |
Line 10: |
| {{STRUCTURE_1oij| PDB=1oij | SCENE= }} | | {{STRUCTURE_1oij| PDB=1oij | SCENE= }} |
|
| |
|
| '''CRYSTAL STRUCTURE OF THE ALKYLSULFATASE ATSK, A NON-HEME FE (II) ALPHAKETOGLUTARATE DEPENDENT DIOXYGENASE IN COMPLEX WITH ALPHAKETOGLUTARATE'''
| | ===CRYSTAL STRUCTURE OF THE ALKYLSULFATASE ATSK, A NON-HEME FE (II) ALPHAKETOGLUTARATE DEPENDENT DIOXYGENASE IN COMPLEX WITH ALPHAKETOGLUTARATE=== |
|
| |
|
|
| |
|
| ==Overview==
| | <!-- |
| The alkylsulfatase AtsK from Pseudomonas putida S-313 belongs to the widespread and versatile non-heme iron(II) alpha-ketoglutarate-dependent dioxygenase superfamily and catalyzes the oxygenolytic cleavage of a variety of different alkyl sulfate esters to the corresponding aldehyde and sulfate. The enzyme is only expressed under sulfur starvation conditions, providing a selective advantage for bacterial growth in soils and rhizosphere. Here we describe the crystal structure of AtsK in the apo form and in three complexes: with the cosubstrate alpha-ketoglutarate, with alpha-ketoglutarate and iron, and finally with alpha-ketoglutarate, iron, and an alkyl sulfate ester used as substrate in catalytic studies. The overall fold of the enzyme is closely related to that of the taurine/alpha-ketoglutarate dioxygenase TauD and is similar to the fold observed for other members of the enzyme superfamily. From comparison of these structures with the crystal structure of AtsK and its complexes, we propose a general mechanism for the catalytic cycle of the alpha-ketoglutarate-dependent dioxygenase superfamily.
| | The line below this paragraph, {{ABSTRACT_PUBMED_15023059}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 15023059 is the PubMed ID number. |
| | --> |
| | {{ABSTRACT_PUBMED_15023059}} |
|
| |
|
| ==About this Structure== | | ==About this Structure== |
| Line 31: |
Line 35: |
| [[Category: Alkylsulfatase]] | | [[Category: Alkylsulfatase]] |
| [[Category: Jelly roll]] | | [[Category: Jelly roll]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 03:53:29 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 09:18:38 2008'' |
