1on7: Difference between revisions

From Proteopedia
Jump to navigationJump to search
← Older editNewer edit →
OCA (talk | contribs)
Bots, Bureaucrats, journal, Administrators
3,331,613 edits
No edit summary
OCA (talk | contribs)
Bots, Bureaucrats, journal, Administrators
3,331,613 edits
No edit summary
Line 1: Line 1:
[[Image:1on7.jpg|left|200px]]
{{Seed}}
[[Image:1on7.png|left|200px]]


<!--
<!--
Line 9: Line 10:
{{STRUCTURE_1on7|  PDB=1on7  |  SCENE=  }}  
{{STRUCTURE_1on7|  PDB=1on7  |  SCENE=  }}  


'''Unmethylated form of C-phycocyanin from Themosynechococcus vulcanus at 2.7A'''
===Unmethylated form of C-phycocyanin from Themosynechococcus vulcanus at 2.7A===




==Overview==
<!--
A novel fraction of c-phycocyanin from the thermophilic cyanobacterium Thermosynechcoccus vulcanus, with an absorption maxima blue-shifted to 612 nm (PC612), has been purified from allophycocyanin and crystallized. The crystals belong to the P63 space group with cell dimensions of 153 A x 153 A x 59 A with a single (alphabeta) monomer in the asymmetric unit, resulting in a solvent content of 65%, and diffract to 2.7 A. The PC612 crystal structure has been determined by molecular replacement and refined to a crystallographic R-factor of 20.9% (Rfree = 27.8%). The crystal packing in this form shows that the PC612 form of phycocyanin does not associate into hexamers and that its association with adjacent trimers in the unit cell is very different from that found in a previously determined structure of the normal form of T. vulcanus phycocyanin, which absorbs at 620 nm. Analysis of the PC612 structure shows that the alpha subunits, which typically form the interface between two trimers within a hexamer, have a high degree of flexibility, as indicated by elevated B-factors in portions of helices B, E, and G. Examination of calculated electron density omit maps shows that unlike all other structures of phycobiliproteins determined so far, the Asnbeta72 residue is not methylated, explaining the blue-shift in its absorption spectra. On the basis of the results presented here, we suggest that this new form of trimeric phycocyanin may constitute a special minor component of the phycobilisome and may form the contact between the phycocyanin rods and the allophycocyanin core.
The line below this paragraph, {{ABSTRACT_PUBMED_12709431}}, adds the Publication Abstract to the page
(as it appears on PubMed at http://www.pubmed.gov), where 12709431 is the PubMed ID number.
-->
{{ABSTRACT_PUBMED_12709431}}


==About this Structure==
==About this Structure==
Line 28: Line 32:
[[Category: Photosynthesis]]
[[Category: Photosynthesis]]
[[Category: Phycobilisome]]
[[Category: Phycobilisome]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 04:03:23 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 21:14:52 2008''

Revision as of 18:14, 27 July 2008

File:1on7.png

Template:STRUCTURE 1on7

Unmethylated form of C-phycocyanin from Themosynechococcus vulcanus at 2.7A

Template:ABSTRACT PUBMED 12709431

About this Structure

1ON7 is a Protein complex structure of sequences from Thermosynechococcus vulcanus. Full crystallographic information is available from OCA.

Reference

The crystal structure of a novel unmethylated form of C-phycocyanin, a possible connector between cores and rods in pycobilisomes., Adir N, Lerner N, J Biol Chem. 2003 Jul 11;278(28):25926-32. Epub 2003 Apr 22. PMID:12709431

Page seeded by OCA on Sun Jul 27 21:14:52 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "https://proteopedia.org/index.php?title=1on7&oldid=667393"