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[[Image:1onj.gif|left|200px]]
{{Seed}}
[[Image:1onj.png|left|200px]]


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{{STRUCTURE_1onj|  PDB=1onj  |  SCENE=  }}  
{{STRUCTURE_1onj|  PDB=1onj  |  SCENE=  }}  


'''Crystal structure of Atratoxin-b from Chinese cobra venom of Naja atra'''
===Crystal structure of Atratoxin-b from Chinese cobra venom of Naja atra===




==Overview==
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Atratoxin-b, a short-chain alpha-neurotoxin purified from Naja atra (mainland Chinese cobra) venom using a three-step chromatography procedure, has an apparent molecular mass of 6950 Da with an alkaline pI value (&gt;9.5) and consists of one single polypeptide chain as estimated by MALDI-TOF mass spectrometry and SDS-PAGE. The protein is toxic to mice, with an in vitro LD(50) of about 0.18 mg kg(-1). Its N-terminal amino-acid sequence, LECHNQQSSQTPTIT, displays a very high homology to those of other alpha-neurotoxins. The overall three-dimensional structure of atratoxin-b is very similar to that of the homologous erabutoxin-a, as shown by the crystallographic molecular replacement and preliminary refinement results, with an R factor and R(free) of 27 and 29%, respectively. The microcrystal slowly grew to dimensions of approximate 0.1 x 0.1 x 0.15 mm over eight months using hanging-drop vapour-diffusion method. It gave a set of diffraction data to 1.56 A resolution using X-rays of wavelength 1.1516 A generated by the X-ray Diffraction and Scattering Station of beamline U7B at the National Synchrotron Radiation Laboratory (Hefei, China); this is the first example of the use of this beamline in protein crystallography. The crystals belong to the tetragonal space group P4(1)2(1)2, with unit-cell parameters a = 49.28, c = 44.80 A, corresponding to one molecule per asymmetric unit and a volume-to-mass ratio of 1.96 A(3) Da(-1).
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{{ABSTRACT_PUBMED_12777767}}


==About this Structure==
==About this Structure==
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[[Category: Beta-turn-beta]]
[[Category: Beta-turn-beta]]
[[Category: Three-finger protein]]
[[Category: Three-finger protein]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 04:04:04 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 15:47:41 2008''

Revision as of 12:47, 27 July 2008

File:1onj.png

Template:STRUCTURE 1onj

Crystal structure of Atratoxin-b from Chinese cobra venom of Naja atra

Template:ABSTRACT PUBMED 12777767

About this Structure

1ONJ is a Single protein structure of sequence from Naja atra. Full crystallographic information is available from OCA.

Reference

Purification, N-terminal sequencing, crystallization and preliminary structural determination of atratoxin-b, a short-chain alpha-neurotoxin from Naja atra venom., Lou X, Tu X, Pan G, Xu C, Fan R, Lu W, Deng W, Rao P, Teng M, Niu L, Acta Crystallogr D Biol Crystallogr. 2003 Jun;59(Pt 6):1038-42. Epub 2003, May 23. PMID:12777767

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