1oo3: Difference between revisions

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[[Image:1oo3.gif|left|200px]]
{{Seed}}
[[Image:1oo3.png|left|200px]]


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{{STRUCTURE_1oo3|  PDB=1oo3  |  SCENE=  }}  
{{STRUCTURE_1oo3|  PDB=1oo3  |  SCENE=  }}  


'''P395S mutant of the p85 regulatory subunit of the N-terminal src homology 2 domain of PI3-Kinase'''
===P395S mutant of the p85 regulatory subunit of the N-terminal src homology 2 domain of PI3-Kinase===




==Overview==
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Understanding the specificity of Src homology 2 (SH2) domains is important because of their critical role in cell signaling. Previous genetic analysis has characterized mutants of the N-terminal src homology 2 (SH2) domain of the p85 subunit of phosphoinositide 3-kinase (PI3K). The P395S mutant exhibits a specificity for phosphopeptide binding different from that of the wild-type SH2. The P395S mutant has an increased affinity for the platelet-derived growth factor receptor (PDGFr) compared to polyomavirus middle T antigen (MT). Solution structures of the P395S mutant of the p85 N-SH2 alone and complexed to a PDGFr phosphopeptide were determined to explain the change in specificity. Chemical shift perturbations caused by different peptides were compared for mutant and wild-type structures. The results show that the single P395S mutation has broad effects on the structure. Furthermore, they provide a rationale for the observed changes in binding preference.
The line below this paragraph, {{ABSTRACT_PUBMED_14503862}}, adds the Publication Abstract to the page
(as it appears on PubMed at http://www.pubmed.gov), where 14503862 is the PubMed ID number.
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{{ABSTRACT_PUBMED_14503862}}


==About this Structure==
==About this Structure==
1OO3 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OO3 OCA].  
1OO3 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OO3 OCA].  


==Reference==
==Reference==
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[[Category: Weyrauch, B.]]
[[Category: Weyrauch, B.]]
[[Category: Src homology 2 domain p85 regulatory subunit mutant]]
[[Category: Src homology 2 domain p85 regulatory subunit mutant]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 04:05:24 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 12:32:32 2008''

Revision as of 09:32, 28 July 2008

File:1oo3.png

Template:STRUCTURE 1oo3

P395S mutant of the p85 regulatory subunit of the N-terminal src homology 2 domain of PI3-Kinase

Template:ABSTRACT PUBMED 14503862

About this Structure

1OO3 is a Single protein structure of sequence from Bos taurus. Full experimental information is available from OCA.

Reference

Nuclear magnetic resonance structure of the P395S mutant of the N-SH2 domain of the p85 subunit of PI3 kinase: an SH2 domain with altered specificity., Gunther UL, Weyrauch B, Zhang X, Schaffhausen B, Biochemistry. 2003 Sep 30;42(38):11120-7. PMID:14503862

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