1orr: Difference between revisions

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[[Image:1orr.jpg|left|200px]]
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[[Image:1orr.png|left|200px]]


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{{STRUCTURE_1orr|  PDB=1orr  |  SCENE=  }}  
{{STRUCTURE_1orr|  PDB=1orr  |  SCENE=  }}  


'''Crystal Structure of CDP-Tyvelose 2-Epimerase complexed with NAD and CDP'''
===Crystal Structure of CDP-Tyvelose 2-Epimerase complexed with NAD and CDP===




==Overview==
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Tyvelose epimerase catalyzes the last step in the biosynthesis of tyvelose by converting CDP-d-paratose to CDP-d-tyvelose. This unusual 3,6-dideoxyhexose occurs in the O-antigens of some types of Gram-negative bacteria. Here we describe the cloning, protein purification, and high-resolution x-ray crystallographic analysis of tyvelose epimerase from Salmonella typhi complexed with CDP. The enzyme from S. typhi is a homotetramer with each subunit containing 339 amino acid residues and a tightly bound NAD+ cofactor. The quaternary structure of the enzyme displays 222 symmetry and can be aptly described as a dimer of dimers. Each subunit folds into two distinct lobes: the N-terminal motif responsible for NAD+ binding and the C-terminal region that harbors the binding site for CDP. The analysis described here demonstrates that tyvelose epimerase belongs to the short-chain dehydrogenase/reductase superfamily of enzymes. Indeed, its active site is reminiscent to that observed for UDP-galactose 4-epimerase, an enzyme that plays a key role in galactose metabolism. Unlike UDP-galactose 4-epimerase where the conversion of configuration occurs about C-4 of the UDP-glucose or UDP-galactose substrates, in the reaction catalyzed by tyvelose epimerase, the inversion of stereochemistry occurs at C-2. On the basis of the observed binding mode for CDP, it is possible to predict the manner in which the substrate, CDP-paratose, and the product, CDP-tyvelose, might be accommodated within the active site of tyvelose epimerase.
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{{ABSTRACT_PUBMED_12642575}}


==About this Structure==
==About this Structure==
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[[Category: Rossmann fold]]
[[Category: Rossmann fold]]
[[Category: Short-chain dehydrogenase/reductase]]
[[Category: Short-chain dehydrogenase/reductase]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 04:12:13 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 18:36:58 2008''

Revision as of 15:36, 28 July 2008

File:1orr.png

Template:STRUCTURE 1orr

Crystal Structure of CDP-Tyvelose 2-Epimerase complexed with NAD and CDP

Template:ABSTRACT PUBMED 12642575

About this Structure

1ORR is a Single protein structure of sequence from Salmonella typhi. Full crystallographic information is available from OCA.

Reference

High resolution x-ray structure of tyvelose epimerase from Salmonella typhi., Koropatkin NM, Liu HW, Holden HM, J Biol Chem. 2003 Jun 6;278(23):20874-81. Epub 2003 Mar 17. PMID:12642575

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