1pio: Difference between revisions

From Proteopedia
Jump to navigationJump to search
← Older editNewer edit →
OCA (talk | contribs)
Bots, Bureaucrats, journal, Administrators
3,330,058 edits
No edit summary
OCA (talk | contribs)
Bots, Bureaucrats, journal, Administrators
3,330,058 edits
No edit summary
Line 1: Line 1:
[[Image:1pio.jpg|left|200px]]
{{Seed}}
[[Image:1pio.png|left|200px]]


<!--
<!--
Line 9: Line 10:
{{STRUCTURE_1pio|  PDB=1pio  |  SCENE=  }}  
{{STRUCTURE_1pio|  PDB=1pio  |  SCENE=  }}  


'''AN ENGINEERED STAPHYLOCOCCUS AUREUS PC1 BETA-LACTAMASE THAT HYDROLYSES THIRD GENERATION CEPHALOSPORINS'''
===AN ENGINEERED STAPHYLOCOCCUS AUREUS PC1 BETA-LACTAMASE THAT HYDROLYSES THIRD GENERATION CEPHALOSPORINS===




==Overview==
<!--
The beta-lactamase from Staphylococcus aureus PC1 has been cloned into an Escherichia coli vector for site-directed mutagenesis and high-level protein expression. A mutant enzyme has been produced in which Ala238 is replaced by a serine, and Ile239 is deleted (A238S:I239del). The engineered enzyme hydrolyses third-generation cephalosporins substantially more rapidly than the parental enzyme does, while hydrolysis of benzylpenicillin is slower with the mutant than with the wild-type and native enzymes. The mutant beta-lactamase has been crystallized and the structure determined and refined at 2.8 A resolution. The disposition of the beta-strand which forms the side of the active site is altered in comparison with the native S. aureus beta-lactamase structure, widening the active site cleft and providing space to accommodate the bulky side-chains of the third-generation cephalosporins.
The line below this paragraph, {{ABSTRACT_PUBMED_8869640}}, adds the Publication Abstract to the page
(as it appears on PubMed at http://www.pubmed.gov), where 8869640 is the PubMed ID number.
-->
{{ABSTRACT_PUBMED_8869640}}


==About this Structure==
==About this Structure==
Line 25: Line 29:
[[Category: Herzberg, O.]]
[[Category: Herzberg, O.]]
[[Category: Zawadzke, L E.]]
[[Category: Zawadzke, L E.]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 05:07:35 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 18:55:19 2008''

Revision as of 15:55, 27 July 2008

File:1pio.png

Template:STRUCTURE 1pio

AN ENGINEERED STAPHYLOCOCCUS AUREUS PC1 BETA-LACTAMASE THAT HYDROLYSES THIRD GENERATION CEPHALOSPORINS

Template:ABSTRACT PUBMED 8869640

About this Structure

1PIO is a Single protein structure of sequence from Staphylococcus aureus. Full crystallographic information is available from OCA.

Reference

An engineered Staphylococcus aureus PC1 beta-lactamase that hydrolyses third-generation cephalosporins., Zawadzke LE, Smith TJ, Herzberg O, Protein Eng. 1995 Dec;8(12):1275-85. PMID:8869640

Page seeded by OCA on Sun Jul 27 18:55:19 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "https://proteopedia.org/index.php?title=1pio&oldid=661785"