1rfa: Difference between revisions
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New page: left|200px<br /> <applet load="1rfa" size="450" color="white" frame="true" align="right" spinBox="true" caption="1rfa" /> '''NMR SOLUTION STRUCTURE OF THE RAS-BINDING D... |
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[[Image:1rfa.gif|left|200px]]<br /> | [[Image:1rfa.gif|left|200px]]<br /><applet load="1rfa" size="350" color="white" frame="true" align="right" spinBox="true" | ||
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'''NMR SOLUTION STRUCTURE OF THE RAS-BINDING DOMAIN OF C-RAF-1'''<br /> | '''NMR SOLUTION STRUCTURE OF THE RAS-BINDING DOMAIN OF C-RAF-1'''<br /> | ||
==Overview== | ==Overview== | ||
The structure of the Ras-binding domain of human c-Raf-1 (residues 55-132) | The structure of the Ras-binding domain of human c-Raf-1 (residues 55-132) has been determined in solution by nuclear magnetic resonance (NMR) spectroscopy. Following complete assignment of the backbone and side-chain 1H, 15N, and 13C resonances, the structure was calculated using the program CHARMM. Over 1300 NOE-derived constraints were applied, resulting in a detailed structure. The fold of Raf55-132 consists of a five-stranded beta-sheet, a 12-residue alpha-helix, and an additional one-turn helix. It is similar to those of ubiquitin and the IgG-binding domain of protein G, although the three proteins share very little sequence identity. The surface of Raf55-132 that interacts with Ras has been identified by monitoring perturbation of line widths and chemical shifts of 15N-labeled Raf55-132 resonances during titration with unlabeled Ras-GMPPNP. The Ras-binding site is contained within a spatially contiguous patch comprised of the N-terminal beta-hairpin and the C-terminal end of the alpha-helix. | ||
==Disease== | |||
Known diseases associated with this structure: LEOPARD syndrome 2 OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=164760 164760]], Noonan syndrome 5 OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=164760 164760]] | |||
==About this Structure== | ==About this Structure== | ||
1RFA is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http:// | 1RFA is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RFA OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Emerson, S | [[Category: Emerson, S D.]] | ||
[[Category: Fry, D | [[Category: Fry, D C.]] | ||
[[Category: Kiefer, S | [[Category: Kiefer, S E.]] | ||
[[Category: Liu, S | [[Category: Liu, S P.]] | ||
[[Category: Madison, V | [[Category: Madison, V S.]] | ||
[[Category: Palermo, R | [[Category: Palermo, R E.]] | ||
[[Category: Scheffler, J | [[Category: Scheffler, J E.]] | ||
[[Category: Tsao, K | [[Category: Tsao, K L.]] | ||
[[Category: Waugh, D | [[Category: Waugh, D S.]] | ||
[[Category: serine/threonine-protein kinase]] | [[Category: serine/threonine-protein kinase]] | ||
[[Category: signal transduction protein]] | [[Category: signal transduction protein]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:50:15 2008'' | ||
