1rj8: Difference between revisions

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[[Image:1rj8.gif|left|200px]]<br />

[[Image:1rj8.gif|left|200px]]<br /><applet load="1rj8" size="350" color="white" frame="true" align="right" spinBox="true"

<applet load="1rj8" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

caption="1rj8, resolution 2.23Å" />

'''The crystal structure of TNF family member EDA-A2'''<br />

==Overview==

EDA is a tumor necrosis factor family member involved in ectodermal, development. Splice variants EDA-A1 and EDA-A2 differ only by the presence, of Glu 308 and Val 309 in the expected receptor binding region of EDA-A1, but not EDA-A2. This two amino acid difference functions as a switch, controlling receptor specificity. EDA-A1 binds only to EDAR, while EDA-A2, is specific for XEDAR. In order to understand the structural basis of this, switch, we determined the X-ray crystal structures of the TNF domain of, both EDA-A1 and EDA-A2 at 2.3 A and 2.2 A, respectively. While the, backbone conformation around the splice difference is similar in both, isoforms, the conformation of the following loop, the surface charge, and, the shape of the expected receptor binding site differ significantly.

EDA is a tumor necrosis factor family member involved in ectodermal development. Splice variants EDA-A1 and EDA-A2 differ only by the presence of Glu 308 and Val 309 in the expected receptor binding region of EDA-A1 but not EDA-A2. This two amino acid difference functions as a switch controlling receptor specificity. EDA-A1 binds only to EDAR, while EDA-A2 is specific for XEDAR. In order to understand the structural basis of this switch, we determined the X-ray crystal structures of the TNF domain of both EDA-A1 and EDA-A2 at 2.3 A and 2.2 A, respectively. While the backbone conformation around the splice difference is similar in both isoforms, the conformation of the following loop, the surface charge, and the shape of the expected receptor binding site differ significantly.

==Disease==

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==About this Structure==

1RJ8 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=1RJ8 OCA].

1RJ8 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RJ8 OCA].

==Reference==

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[[Category: Single protein]]

[[Category: Ackerly, H.]]

[[Category: Compaan, D.M.]]

[[Category: Compaan, D M.]]

[[Category: Dixit, V.M.]]

[[Category: Dixit, V M.]]

[[Category: Hymowitz, S.G.]]

[[Category: Hymowitz, S G.]]

[[Category: Starovasnik, M.A.]]

[[Category: Starovasnik, M A.]]

[[Category: Vos, A.M.de.]]

[[Category: Vos, A M.de.]]

[[Category: Yan, M.]]

[[Category: jelly roll]]

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[[Category: trimer]]

''Page seeded by [http://~~ispc~~.weizmann.ac.il/oca OCA ] on ~~Mon Nov 12 19~~:04:~~23 2007~~''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:51:39 2008''

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@@ Line 1: / Line 1: @@
-[[Image:1rj8.gif|left|200px]]<br />
+[[Image:1rj8.gif|left|200px]]<br /><applet load="1rj8" size="350" color="white" frame="true" align="right" spinBox="true"
-<applet load="1rj8" size="450" color="white" frame="true" align="right" spinBox="true"
 caption="1rj8, resolution 2.23&Aring;" />
 '''The crystal structure of TNF family member EDA-A2'''<br />
 ==Overview==
-EDA is a tumor necrosis factor family member involved in ectodermal, development. Splice variants EDA-A1 and EDA-A2 differ only by the presence, of Glu 308 and Val 309 in the expected receptor binding region of EDA-A1, but not EDA-A2. This two amino acid difference functions as a switch, controlling receptor specificity. EDA-A1 binds only to EDAR, while EDA-A2, is specific for XEDAR. In order to understand the structural basis of this, switch, we determined the X-ray crystal structures of the TNF domain of, both EDA-A1 and EDA-A2 at 2.3 A and 2.2 A, respectively. While the, backbone conformation around the splice difference is similar in both, isoforms, the conformation of the following loop, the surface charge, and, the shape of the expected receptor binding site differ significantly.
+EDA is a tumor necrosis factor family member involved in ectodermal development. Splice variants EDA-A1 and EDA-A2 differ only by the presence of Glu 308 and Val 309 in the expected receptor binding region of EDA-A1 but not EDA-A2. This two amino acid difference functions as a switch controlling receptor specificity. EDA-A1 binds only to EDAR, while EDA-A2 is specific for XEDAR. In order to understand the structural basis of this switch, we determined the X-ray crystal structures of the TNF domain of both EDA-A1 and EDA-A2 at 2.3 A and 2.2 A, respectively. While the backbone conformation around the splice difference is similar in both isoforms, the conformation of the following loop, the surface charge, and the shape of the expected receptor binding site differ significantly.
 ==Disease==
@@ Line 11: / Line 10: @@
 ==About this Structure==
-RJ8 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1RJ8 OCA].
+RJ8 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RJ8 OCA].
 ==Reference==
@@ Line 18: / Line 17: @@
 [[Category: Single protein]]
 [[Category: Ackerly, H.]]
-[[Category: Compaan, D.M.]]
+[[Category: Compaan, D M.]]
-[[Category: Dixit, V.M.]]
+[[Category: Dixit, V M.]]
-[[Category: Hymowitz, S.G.]]
+[[Category: Hymowitz, S G.]]
-[[Category: Starovasnik, M.A.]]
+[[Category: Starovasnik, M A.]]
-[[Category: Vos, A.M.de.]]
+[[Category: Vos, A M.de.]]
 [[Category: Yan, M.]]
 [[Category: jelly roll]]
@@ Line 29: / Line 28: @@
 [[Category: trimer]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 12 19:04:23 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:51:39 2008''