1ptk: Difference between revisions

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[[Image:1ptk.jpg|left|200px]]
{{Seed}}
[[Image:1ptk.png|left|200px]]


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{{STRUCTURE_1ptk|  PDB=1ptk  |  SCENE=  }}  
{{STRUCTURE_1ptk|  PDB=1ptk  |  SCENE=  }}  


'''STUDIES ON THE INHIBITORY ACTION OF MERCURY UPON PROTEINASE K'''
===STUDIES ON THE INHIBITORY ACTION OF MERCURY UPON PROTEINASE K===




==Overview==
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In proteinase K, Cys73 is located "below" the imidazole of the active site His69. In a 2.4-A resolution x-ray crystal structure of the complex formed between the enzyme and HgAc2, two Hg(II) positions are found: a fully occupied site, covalently bound to Cys73 (S gamma), which disrupts the catalytic triad (Asp39-His69-Ser224), and a 2-fold disordered (25 and 35% occupancy), noncovalent complexation to His72, Cys73, and Thr76 of lower affinity. The enzyme is inhibited noncompetitively at low concentrations and competitively above stoichiometric concentrations of Hg(II), but it retains 7% residual activity. This can be rationalized if the molecule is flexible enough to permit transient formation of the catalytic triad. Except for the active site, only minor structural changes are observed upon binding of Hg(II), but the thermal stability is reduced by 4 degrees C.
The line below this paragraph, {{ABSTRACT_PUBMED_8253733}}, adds the Publication Abstract to the page
(as it appears on PubMed at http://www.pubmed.gov), where 8253733 is the PubMed ID number.
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{{ABSTRACT_PUBMED_8253733}}


==About this Structure==
==About this Structure==
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[[Category: Mueller, A.]]
[[Category: Mueller, A.]]
[[Category: Saenger, W.]]
[[Category: Saenger, W.]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 05:28:16 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 23:23:09 2008''

Revision as of 20:23, 27 July 2008

File:1ptk.png

Template:STRUCTURE 1ptk

STUDIES ON THE INHIBITORY ACTION OF MERCURY UPON PROTEINASE K

Template:ABSTRACT PUBMED 8253733

About this Structure

1PTK is a Single protein structure of sequence from Engyodontium album. Full crystallographic information is available from OCA.

Reference

Studies on the inhibitory action of mercury upon proteinase K., Muller A, Saenger W, J Biol Chem. 1993 Dec 15;268(35):26150-4. PMID:8253733

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