6zq3

Crystal Structure of Silicatein Alpha from Marine Sponge Tethya aurantium

spinqualitylabelsall models 6zq3, resolution 2.40Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2.4Å Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed Formation of highly symmetric skeletal elements in demosponges, called spicules, follows a unique biomineralization mechanism in which polycondensation of an inherently disordered amorphous silica is guided by a highly ordered proteinaceous scaffold, the axial filament. The enzymatically active proteins, silicateins, are assembled into a slender hybrid silica/protein crystalline superstructure that directs the morphogenesis of the spicules. Furthermore, silicateins are known to catalyze the formation of a large variety of other technologically relevant organic and inorganic materials. However, despite the biological and biotechnological importance of this macromolecule, its tertiary structure was never determined. Here we report the atomic structure of silicatein and the entire mineral/organic hybrid assembly with a resolution of 2.4 A. In this work, the serial X-ray crystallography method was successfully adopted to probe the 2-microm-thick filaments in situ, being embedded inside the skeletal elements. In combination with imaging and chemical analysis using high-resolution transmission electron microscopy, we provide detailed information on the enzymatic activity of silicatein, its crystallization, and the emergence of a functional three-dimensional silica/protein superstructure in vivo. Ultimately, we describe a naturally occurring mineral/protein crystalline assembly at atomic resolution. Natural hybrid silica/protein superstructure at atomic resolution.,Gorlich S, Samuel AJ, Best RJ, Seidel R, Vacelet J, Leonarski FK, Tomizaki T, Rellinghaus B, Pohl D, Zlotnikov I Proc Natl Acad Sci U S A. 2020 Dec 8;117(49):31088-31093. doi:, 10.1073/pnas.2019140117. Epub 2020 Nov 23. PMID:33229574 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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