7okv

Crystal structure of soluble EPCR after exposure to the nonionic surfactant Polysorbate 20

spinqualitylabelsall models 7okv, resolution 1.85Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 7okv is a 1 chain structure with sequence from Homo sapiens. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 1.85Å Ligands: D10, EDO, HEX, NAG, R16 Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function EPCR_HUMAN Binds activated protein C. Enhances protein C activation by the thrombin-thrombomodulin complex; plays a role in the protein C pathway controlling blood coagulation. Publication Abstract from PubMed Evidence is mounting that the nature of the lipid bound to the endothelial cell protein C receptor (EPCR) has an impact on its biological roles, as observed in anticoagulation and more recently, in autoimmune disease. Phosphatidylethanolamine and phosphatidylcholine species dominate the EPCR lipid cargo, yet, the extent of diversity in the EPCR-associated lipid repertoire is still unknown and remains to be uncovered. We undertook mass spectrometry analyses to decipher the EPCR lipidome, and identified species not yet described as EPCR ligands, such as phosphatidylinositols and phosphatidylserines. Remarkably, we found further, more structurally divergent lipids classes, represented by ceramides and sphingomyelins, both in less abundant quantities. In support of our mass spectrometry results and previous studies, high-resolution crystal structures of EPCR in three different space groups point to a prevalent diacyl phospholipid moiety in EPCR's pocket but a mobile and ambiguous lipid polar head group. In sum, these studies indicate that EPCR can associate with varied lipid classes, which might impact its properties in anticoagulation and the onset of autoimmune disease. Identification of a broad lipid repertoire associated to the endothelial cell protein C receptor (EPCR).,Erausquin E, Moran-Garrido M, Saiz J, Barbas C, Dichiara-Rodriguez G, Urdiciain A, Lopez-Sagaseta J Sci Rep. 2022 Sep 6;12(1):15127. doi: 10.1038/s41598-022-18844-y. PMID:36068249 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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